Low-cost, high-throughput and rapid-prototyped 3D-integrated dielectrophoretic channels for continuous cell enrichment and separation.
Electrophoresis
; 44(11-12): 947-955, 2023 Jun.
Article
em En
| MEDLINE
| ID: mdl-36409835
ABSTRACT
Microfluidic devices for dielectrophoretic cell separation are typically designed and constructed using microfabrication methods in a clean room, requiring time and expense. In this paper, we describe a novel alternative approach to microfluidic device manufacture, using chips cut from conductor-insulator laminates using a cutter plotter. This allows the manufacture of microchannel devices with micron-scale electrodes along every wall. Fabrication uses a conventional desktop cutter plotter, and requires no chemicals, masks or clean-room access; functional fluidic devices can be designed and constructed within a couple of hours at negligible cost. As an example, we demonstrate the construction of a continuous dielectrophoretic cell separator capable of enriching yeast cells to 80% purity at 10â¯000 cells/s.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microfluídica
/
Técnicas Analíticas Microfluídicas
Tipo de estudo:
Health_economic_evaluation
Idioma:
En
Revista:
Electrophoresis
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Reino Unido