Crystal Structure of the Metallo-Endoribonuclease YbeY from Staphylococcus aureus.
J Microbiol Biotechnol
; 33(1): 28-34, 2023 Jan 28.
Article
em En
| MEDLINE
| ID: mdl-36457189
ABSTRACT
Endoribonuclease YbeY is specific to the single-stranded RNA of ribosomal RNAs and small RNAs. This enzyme is essential for the maturation and quality control of ribosomal RNA in a wide range of bacteria and for virulence in some pathogenic bacteria. In this study, we determined the crystal structure of YbeY from Staphylococcus aureus at a resolution of 1.9 Å in the presence of zinc chloride. The structure showed a zinc ion at the active site and two molecules of tricarboxylic acid citrate, which were also derived from the crystallization conditions. Our structure showed the zinc ion-bound local environment at the molecular level for the first time. Molecular comparisons were performed between the carboxylic moieties of citrate and the phosphate moiety of the RNA backbone, and a model of YbeY in complex with a single strand of RNA was subsequently constructed. Our findings provide molecular insights into how the YbeY enzyme recognizes single-stranded RNA in bacteria.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Staphylococcus aureus
/
Endorribonucleases
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Microbiol Biotechnol
Ano de publicação:
2023
Tipo de documento:
Article