Geographical and seasonal phytochemical variation of Artemisia afra Jacq. ex Willd.
Phytochem Anal
; 34(2): 175-185, 2023 Mar.
Article
em En
| MEDLINE
| ID: mdl-36464634
ABSTRACT
INTRODUCTION:
Artemisia afra Jacq. ex. Willd. (Asteraceae) is a popular traditional medicine in South Africa, mainly used in the form of an infusion, for the treatment of respiratory ailments. Quality control methods are limited and phytochemical variation for the infusion is not well known.OBJECTIVE:
To develop a sensitive quality control method for A. afra infusions by validating a liquid chromatography electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS) method and quantitatively comparing six marker compounds in A. afra samples collected from different locations and over a 12-month period. MATERIAL ANDMETHODS:
There was a multiple reaction monitoring method optimised and validated, according to ICH and FDA guidelines, to quantify the chemical markers present in infusions.RESULTS:
The chemistry differed significantly and interestingly, with an interchangeable trend between chlorogenic acid (CGA) and 4,5-dicaffeoylquinic acid (DCQA) observed in the samples collected monthly, elevated levels of CGA during winter and elevated levels of DCQA during summer. The remaining four markers showed a steady decrease as winter approached and a steady increase as summer approached. The ranges of the six markers were the following CGA (0.68-14.68 µg/mg), DCQA (0.005-8.110 µg/mg), quercetin (0.01-0.65 µg/mg), luteolin (0.05-1.30 ng/mg), scopoletin (0.10-1.14 µg/mg), scopolin (0.03-1.21 µg/mg).CONCLUSIONS:
A sensitive LC-ESI-MS/MS method was developed, validated, and used to quantify six marker compounds. The results indicated a large degree of phytochemical variation occurred across all samples tested, which highlights the importance of producing herbal medicine under controlled conditions and the necessity of analytical quality control methods.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
/
Artemisia
Tipo de estudo:
Guideline
Idioma:
En
Revista:
Phytochem Anal
Assunto da revista:
BOTANICA
/
QUIMICA
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
África do Sul