Rapid saline-alkali sensitivity testing using hydrogel/gold nanoparticles-modified screen-printed electrodes.
Sci Total Environ
; 862: 160814, 2023 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-36509274
ABSTRACT
Rapid screening of microorganisms with good saline-alkali tolerance is of great significance for the improvement of saline-alkali land. In this study, a novel electrochemical method was developed for the rapid screening of saline-alkali-tolerant bacteria using a hydrogel/gold nanoparticles-modified screen-printed electrode. Monitoring bacterial growth using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) yielded a new method to measure saline-alkali sensitivity. The strains were deposited on agarose hydrogel-AuNPs composite-modified electrodes with saline-alkali treatment control at a concentration of 50 mM. The electrochemical-derived growth curve of each bacterial strain was established to monitor the effect of saline-alkaline conditions on bacterial growth. The results showed that E. coli could grow on the hydrogel-AuNPs composite-modified electrodes without saline and alkali, while the growth of E. coli was inhibited after adding saline and alkali to the modified electrodes. In contrast, Paenibacillus lautus (HC_A) and Lysinibacillus fusiformis (HC_B) were able to grow on electrodes containing saline-alkali hydrogel-AuNPs composite modification. This fast growth curves of the strains derived from electrochemical analysis indicate that the possible time for salinity sensitivity results is <45 min. Compared to the traditional bacterial culture method lasting at least 1-2 days, this method has the clear advantages of rapidity, high efficiency, and low cost.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Nanopartículas Metálicas
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Sci Total Environ
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
China