Intranasal delivery of darunavir improves brain drug concentrations in mice for effective HIV treatment.
Biochem Biophys Rep
; 33: 101408, 2023 Mar.
Article
em En
| MEDLINE
| ID: mdl-36532875
ABSTRACT
Despite the availability of combined antiretroviral therapy (cART), which reduces the HIV replication in chronically HIV-infected patients, HIV associated neurocognitive disorders (HAND) persists in the brain. The blood-brain barrier (BBB) is the major barrier for the penetration of drugs including antiretrovirals, limiting the drug penetration to the brain. In the present study, we have shown improved brain drug concentration in mice for darunavir (DRV), an FDA-approved drug, using an intranasal (IN) delivery method that bypasses the BBB. Here, we compared the time-dependent biodistribution of DRV at two different concentrations, high (25 mg/kg) and low (2.5 mg/kg), using two administration routes intravenous (IV) and intranasal (IN) in brain, liver, lungs, and plasma. Compared with IV administration, IN administration demonstrated a significantly improved DRV penetration in the brain at both low and high DRV concentrations (IV vs IN at 2.5 mg/kg 6.91 ± 1.69 ng/g vs 12.08 ± 2.91 ng/g, at 25 mg/kg 12.84 ± 2.88 ng/g vs 19.74 ± 1.80 ng/g). As expected, IN administration showed significantly lower DRV concentrations in plasma (IV vs IN at 2.5 mg/kg 81.37 ± 22.04 ng/g vs 19.91 ± 12.65 ng/g, at 25 mg/kg 899.12 ± 136.93 ng/g vs 320.56 ± 40.04 ng/g) and liver (IV vs IN at 2.5 mg/kg 118.39 ± 28.13 ng/g vs 29.27 ± 4.17 ng/g at 25 mg/kg 1085.18 ± 255.0 ng/g vs 833.83 ± 242.4 ng/g). The IN administration did not show significant change in lungs compared to the IV administration. As a result, these findings suggest that the IN route can increase the DRV level in the brain, suppressing HIV in the brain reservoirs. Additionally, it could also reduce off-target effects, especially in peripheral organs.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Biochem Biophys Rep
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Estados Unidos