Detection of 1-OHPyr in human urine using SERS with injection under wet liquid-liquid self-assembled films of ß-CD-coated gold nanoparticles and deep learning.
Spectrochim Acta A Mol Biomol Spectrosc
; 290: 122238, 2023 Apr 05.
Article
em En
| MEDLINE
| ID: mdl-36592595
ABSTRACT
1-Hydroxypyrene (1-OHPyr), a typical hydroxylated polycyclic aromatic hydrocarbon (OH-PAH), has been commonly regarded as a urinary biomarker for assessing human exposure and health risks of PAHs. Herein, a fast and sensitive method was developed for the determination of 1-OHPyr in urine using surface-enhanced Raman spectroscopy (SERS) combined with deep learning (DL). After emulsification, urinary 1-OHPyr was separated using simple liquid-liquid extraction. Gold nanoparticles with ß-cyclodextrin (ß-CD@AuNPs) were synthesized, and homogeneous and ordered ß-CD@AuNP films were prepared through a liquid-liquid interface self-assembly process. The separated 1-OHPyr was injected under wet assembled films for SERS detection. Concentration as low as 0.05 µg mL-1 of 1-OHPyr in urine could still be detected, and the relative standard deviation was 5.5 %, and this was ascribed to the adsorption of ß-CD and the high-probability contact between 1-OHPyr molecules and the nanogap of assembled films under the action of capillary force. Meanwhile, a convolutional neural network (CNN), a classical DL network architecture, was adopted to build the prediction model, and the model was further simplified by genetic algorithm (GA). CNN combined with a GA obtained optimized results with determination coefficient and a root mean square error of prediction sets of 0.9639 and 0.6327, respectively, outperforming other models. Overall, the proposed method achieves fast and accurate detection of 1-OHPyr in urine, improves the assessment human exposure to PAHs and is expected to have applications in the analysis of other OH-PAHs in complex environments.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hidrocarbonetos Policíclicos Aromáticos
/
Nanopartículas Metálicas
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Aprendizado Profundo
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Spectrochim Acta A Mol Biomol Spectrosc
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2023
Tipo de documento:
Article