Determination of molecular forms of brain acetylcholinesterase: technical considerations.
Brain Res Bull
; 19(2): 287-90, 1987 Aug.
Article
em En
| MEDLINE
| ID: mdl-3664285
ABSTRACT
Sucrose density centrifugation has been used to characterize the relative levels of AChE molecular forms in different parts of the brain, during development, or in various disease states. We have examined the influence of various tissue or sample storage and handling techniques on the abundance of the 4S and 10S molecular forms of AChE in rat forebrain. Our results demonstrate that freezing either a subcellular fraction or the intact tissue causes dramatic shifts in the level of the 4S and 10S molecular forms as compared to the values obtained in fresh tissue. Total AChE activity was unchanged suggesting that 4S and 10S forms are equally active and that 4S AChE is easily dissociated from 10S. These observations suggest that 4S and 10S molecular forms in brain are extremely labile and that great care should be taken when studying the factors that regulate these forms.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Acetilcolinesterase
/
Manejo de Espécimes
/
Encéfalo
Limite:
Animals
Idioma:
En
Revista:
Brain Res Bull
Ano de publicação:
1987
Tipo de documento:
Article