Fluorophore-encapsulated nanobeads for on-site, rapid, and sensitive lateral flow assay.
Sens Actuators B Chem
; 381: 133364, 2023 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-36684645
ABSTRACT
Since December 2019, the rapid and sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a priority for public health. Although the lateral flow assay (LFA) sensor has emerged as a rapid and on-site SARS-CoV-2 detection technique, the conventional approach of using gold nanoparticles for the signaling probe had limitations in increasing the sensitivity of the sensor. Herein, our newly suggested methodology to improve the performance of the LFA system could amplify the sensor signal with a facile fabrication method by concentrating fluorescent organic molecules. A large Stokes shift fluorophore (single benzene) was encapsulated into polystyrene nanobeads to enhance the fluorescence intensity of the probe for LFA sensor, which was detected on the test line with a longpass filter under ultraviolet light irradiation. This approach provides comparatively high sensitivity with the limit of detection of 1 ng mL-1 for the SARS-CoV-2 spike protein and a fast detection process, which takes less than 20 min. Furthermore, our sensor showed higher performance than gold nanoparticle-based commercial rapid diagnostics test kits in clinical tests, proving that this approach is more suitable and reliable for the sensitive and rapid detection of viruses, bacteria, and other hazardous materials.
Ab, Antibody; Ag, Antigen; AuNP, Gold nanoparticle; CL, Control line; CT, Threshold cycle; LFA, Lateral flow assay; LOD, Limit of detection; PCR, Polymerase chain reaction; PS, Polystyrene; RDT, Rapid diagnostic test; SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2; SB, Single Benzene; TL, Test line
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Sens Actuators B Chem
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Coréia do Sul