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Red-Shifted Coumarin Luciferins for Improved Bioluminescence Imaging.
Love, Anna C; Caldwell, Donald R; Kolbaba-Kartchner, Bethany; Townsend, Katherine M; Halbers, Lila P; Yao, Zi; Brennan, Caroline K; Ivanic, Joseph; Hadjian, Tanya; Mills, Jeremy H; Schnermann, Martin J; Prescher, Jennifer A.
Afiliação
  • Love AC; Department of Chemistry, University of California, Irvine, Irvine, California 92697, United States.
  • Caldwell DR; Chemical Biology Laboratory, Cancer for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, United States.
  • Kolbaba-Kartchner B; School of Molecular Sciences, Arizona State University, Tempe, Arizona 85281, United States.
  • Townsend KM; The Biodesign Center for Molecular Design and Biomimetics, Arizona State University, Tempe, Arizona 85281, United States.
  • Halbers LP; Department of Chemistry, University of California, Irvine, Irvine, California 92697, United States.
  • Yao Z; Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, California 92697, United States.
  • Brennan CK; Department of Chemistry, University of California, Irvine, Irvine, California 92697, United States.
  • Ivanic J; Department of Chemistry, University of California, Irvine, Irvine, California 92697, United States.
  • Hadjian T; Advanced Biomedical Computational Science, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc., Frederick, Maryland 21702, United States.
  • Mills JH; Department of Chemistry, University of California, Irvine, Irvine, California 92697, United States.
  • Schnermann MJ; School of Molecular Sciences, Arizona State University, Tempe, Arizona 85281, United States.
  • Prescher JA; The Biodesign Center for Molecular Design and Biomimetics, Arizona State University, Tempe, Arizona 85281, United States.
J Am Chem Soc ; 145(6): 3335-3345, 2023 02 15.
Article em En | MEDLINE | ID: mdl-36745536
ABSTRACT
Multicomponent bioluminescence imaging in vivo requires an expanded collection of tissue-penetrant probes. Toward this end, we generated a new class of near-infrared (NIR) emitting coumarin luciferin analogues (CouLuc-3s). The scaffolds were easily accessed from commercially available dyes. Complementary mutant luciferases for the CouLuc-3 analogues were also identified. The brightest probes enabled sensitive imaging in vivo. The CouLuc-3 scaffolds are also orthogonal to popular bioluminescent reporters and can be used for multicomponent imaging applications. Collectively, this work showcases a new set of bioluminescent tools that can be readily implemented for multiplexed imaging in a variety of biological settings.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Luciferina de Vaga-Lumes / Luciferinas Idioma: En Revista: J Am Chem Soc Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Luciferina de Vaga-Lumes / Luciferinas Idioma: En Revista: J Am Chem Soc Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos