Protocol for analyzing enzymatic hydrolysis of cellulose using surface pitting observation technology.
STAR Protoc
; 4(1): 102066, 2023 03 17.
Article
em En
| MEDLINE
| ID: mdl-36825811
ABSTRACT
Assaying enzymatic degradation of water-insoluble substrates like cellulose is challenging because only the substrate surface is accessible to the enzymes resulting in low reaction rates. Here, we describe a protocol for surface pitting observation technology (SPOT), an ultra-sensitive quantitative assay for analyzing enzymatic hydrolysis of cellulose. We describe the use of a porous substrate to accelerate the hydrolysis rate of cellulose. We also detail the steps for combining inkjet patterning and optical profilometry to analyze volume loss upon hydrolysis. For complete details on the use and execution of this protocol, please refer to Tsudome et al. (2022).1.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tecnologia
/
Celulose
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2023
Tipo de documento:
Article