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Prevalence of HIV-1 Natural Polymorphisms and Integrase-Resistance-Associated Mutations in African Children.
Fofana, Djeneba B; Diarra, Houdou; Guindo, Ibrahima; Savadogo, Mahamadou K; d'Almeida, Marceline; Diallo, Fatoumata I; Baldé, Aliou; Soulié, Cathia; Kone, Amadou; Marcelin, Anne-Geneviève; Maiga, Almoustapha I; Lambert-Niclot, Sidonie; Maiga, Mamoudou; McFall, Sally; Hawkins, Claudia A; Murphy, Robert L; Sylla, Mariam; Katlama, Christine; Holl, Jane L; Calvez, Vincent; Morand-Joubert, Laurence.
Afiliação
  • Fofana DB; Faculty of Medicine, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako E 423, Mali.
  • Diarra H; Sorbonne Université, INSERM, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), F-75012 Paris, France.
  • Guindo I; Department of Virology, Assistance Publique-Hôpitaux de Paris (AP-HP), Saint-Antoine Hospital, F-75012 Paris, France.
  • Savadogo MK; Faculty of Medicine, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako E 423, Mali.
  • d'Almeida M; Faculty of Medicine, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako E 423, Mali.
  • Diallo FI; Centre d'Ecoute, de Soins, d'Animation et de Conseils (CESAC), Bamako E 2561, Mali.
  • Baldé A; Département Mère Enfant, Faculté Des Sciences De La Santé, Université Abomey-Calavi, CNHU-HKM, Cotonou 229, Benin.
  • Soulié C; Faculty of Medicine, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako E 423, Mali.
  • Kone A; Faculty of Medicine, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako E 423, Mali.
  • Marcelin AG; Sorbonne Université, INSERM, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), F-75012 Paris, France.
  • Maiga AI; Sorbonne Université, INSERM, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), F-75012 Paris, France.
  • Lambert-Niclot S; Department of Virology, Assistance Publique-HÔpitaux de Paris (AP-HP), Pitié Salpêtrière Hospital, F-75013 Paris, France.
  • Maiga M; Faculty of Medicine, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako E 423, Mali.
  • McFall S; Sorbonne Université, INSERM, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), F-75012 Paris, France.
  • Hawkins CA; Department of Virology, Assistance Publique-HÔpitaux de Paris (AP-HP), Pitié Salpêtrière Hospital, F-75013 Paris, France.
  • Murphy RL; Faculty of Medicine, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako E 423, Mali.
  • Sylla M; Sorbonne Université, INSERM, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), F-75012 Paris, France.
  • Katlama C; Department of Virology, Assistance Publique-Hôpitaux de Paris (AP-HP), Saint-Antoine Hospital, F-75012 Paris, France.
  • Holl JL; Faculty of Medicine, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako E 423, Mali.
  • Calvez V; Institute for Global Health, Northwestern University, Chicago, IL 60208, USA.
  • Morand-Joubert L; Institute for Global Health, Northwestern University, Chicago, IL 60208, USA.
Viruses ; 15(2)2023 02 16.
Article em En | MEDLINE | ID: mdl-36851760
ABSTRACT
Integrase inhibitors (INIs) are a potent option for HIV treatment. Limited data exist on INI resistance in West Africa, particularly in children living with HIV/AIDS. We determined the prevalence of integrase gene polymorphisms and the frequency of naturally occurring amino acid (aa) substitutions at positions associated with INI resistance. Dried blood spot (DBS) samples were obtained from one hundred and seven (107) HIV-1-infected children aged less than 15 years old in two West African countries, Benin and Mali. All children were naïve to INI treatment, 56 were naïve to anti-retroviral therapy (ART), and 51 had received ART. Genetic sequencing of HIV integrase was successful in 75 samples. The aa changes at integrase positions associated with INI resistance were examined according to the Stanford HIV Genotypic Resistance database. The median ages were 2.6 and 10 years for ART-naïve and -treated children, respectively. The most common subtypes observed were CRF02_AG (74.7%) followed by CRF06_cpx (20%). No major INI-resistance mutations at positions 66, 92, 121, 143, 147, 148, 155, and 263 were detected. The most prevalent INI accessory resistance mutations were L74I/M (14/75, 18.6%) followed by E157Q (8/75, 10.6%), G163E/N/T/Q (5/75, 6.6%), Q95A/H/P (2/75, 2.6%), and T97A (4/75, 5.3%). Other substitutions observed were M50I/L/P, H51E/P/S/Q, I72V, T112V, V201I, and T206S. Polymorphisms at positions which may influence the genetic barrier and/or drive the selection of specific INI-resistance pathways were detected. However, no transmitted drug resistance (TDR) to INI was detected among samples of INI-naïve patients. These findings support the use of this treatment class for children with HIV-1, particularly in West Africa.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: HIV-1 / Soropositividade para HIV / Integrase de HIV Tipo de estudo: Prevalence_studies / Risk_factors_studies Limite: Adolescent / Child / Child, preschool / Humans País/Região como assunto: Africa Idioma: En Revista: Viruses Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Mali

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: HIV-1 / Soropositividade para HIV / Integrase de HIV Tipo de estudo: Prevalence_studies / Risk_factors_studies Limite: Adolescent / Child / Child, preschool / Humans País/Região como assunto: Africa Idioma: En Revista: Viruses Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Mali