Genetic ablation: targeted expression of a toxin gene causes microphthalmia in transgenic mice.
Science
; 238(4833): 1563-5, 1987 Dec 11.
Article
em En
| MEDLINE
| ID: mdl-3685993
ABSTRACT
Lineage-specific regulatory elements can be used to direct expression of a variety of genes to specific tissues in transgenic mice. If the hybrid constructs contain a gene encoding a cytotoxic gene product, then genetic ablation of a specific cell lineage can be achieved. We have generated six transgenic mice by introducing into fertilized eggs the mouse gamma 2-crystallin promoter fused to the coding region of the diphtheria toxin A-chain gene. Three of these mice and all the transgenic offspring analyzed were microphthalmic. The lenses of these mice displayed considerable heterogeneity some were almost normal morphologically but reduced in size, whereas others were grossly aberrant and deficient in nuclear fiber cells. These studies indicate that programmed ablation of specific cell types can be stably transmitted through the germ line.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microftalmia
/
Cristalinas
/
Toxina Diftérica
/
Genes
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Science
Ano de publicação:
1987
Tipo de documento:
Article
País de afiliação:
Canadá