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Zasp52 strengthens whole embryo tissue integrity through supracellular actomyosin networks.
Ashour, Dina J; Durney, Clinton H; Planelles-Herrero, Vicente J; Stevens, Tim J; Feng, James J; Röper, Katja.
Afiliação
  • Ashour DJ; MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK.
  • Durney CH; Department of Mathematics, University of British Columbia, Vancouver, V6T 1Z2Canada.
  • Planelles-Herrero VJ; MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK.
  • Stevens TJ; MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK.
  • Feng JJ; Department of Mathematics, University of British Columbia, Vancouver, V6T 1Z2Canada.
  • Röper K; Department of Chemical and Biological Engineering, University of British Columbia, Vancouver, V6T 1Z3Canada.
Development ; 150(7)2023 04 01.
Article em En | MEDLINE | ID: mdl-36897564
During morphogenesis, large-scale changes of tissue primordia are coordinated across an embryo. In Drosophila, several tissue primordia and embryonic regions are bordered or encircled by supracellular actomyosin cables, junctional actomyosin enrichments networked between many neighbouring cells. We show that the single Drosophila Alp/Enigma-family protein Zasp52, which is most prominently found in Z-discs of muscles, is a component of many supracellular actomyosin structures during embryogenesis, including the ventral midline and the boundary of the salivary gland placode. We reveal that Zasp52 contains within its central coiled-coil region a type of actin-binding motif usually found in CapZbeta proteins, and this domain displays actin-binding activity. Using endogenously-tagged lines, we identify that Zasp52 interacts with junctional components, including APC2, Polychaetoid and Sidekick, and actomyosin regulators. Analysis of zasp52 mutant embryos reveals that the severity of the embryonic defects observed scales inversely with the amount of functional protein left. Large tissue deformations occur where actomyosin cables are found during embryogenesis, and in vivo and in silico analyses suggest a model whereby supracellular Zasp52-containing cables aid to insulate morphogenetic changes from one another.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Actomiosina / Proteínas de Drosophila Limite: Animals Idioma: En Revista: Development Assunto da revista: BIOLOGIA / EMBRIOLOGIA Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Actomiosina / Proteínas de Drosophila Limite: Animals Idioma: En Revista: Development Assunto da revista: BIOLOGIA / EMBRIOLOGIA Ano de publicação: 2023 Tipo de documento: Article