Transcriptome analysis of the Clostridioides difficile response to a specific lactobacilli probiotic formulation: explanations for its mechanisms of action.
J Appl Microbiol
; 134(3)2023 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-36898672
ABSTRACT
AIMS:
Clostridioides difficileâ¯infections (CDI) are a major cause of morbidity and mortality in hospitalized patients. A probiotic formulation (Bio-K+) comprised ofâ¯Lactobacillus acidophilusâ¯CL1285, Lacticaseibacillus caseiâ¯LBC80R, and Lacti. rhamnosusâ¯CLR2 strains have been shown to reduce the incidence of CDI and antibiotic-associated diarrhea (AAD). This research aims to therefore elucidate the mechanism of action ofâ¯the three probiotic strained againstâ¯C. difficile R20291, independently of the acidification of the environment.â¯. METHODS ANDRESULTS:
Antitoxin activity was evaluated using ELISA method and the expression ofâ¯C. difficileâ¯genes was evaluated using transcriptomic analysis in co-culture assays conducted in a bioreactor allowing precise control of the pH. The fermentation results demonstrated a decrease for toxin A and many genes directly related toâ¯C. difficileâ¯virulence were underexpressed in the co-cultures.CONCLUSIONS:
The lactobacilli tested could have a role in the motility, the quorum sensing (QS), the survival of the spores, and the germination potential of the spores, which are essential elements for the virulence ofâ¯C. difficile.â¯.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Clostridioides difficile
/
Infecções por Clostridium
/
Probióticos
Limite:
Humans
Idioma:
En
Revista:
J Appl Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Canadá