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An updated catalogue of diverse type II polyketide synthase biosynthetic gene clusters captured from large-scale nucleotide databases.
McBride, Christina M; Miller, Eric L; Charkoudian, Louise K.
Afiliação
  • McBride CM; Department of Chemistry, Haverford College, Haverford, PA, USA.
  • Miller EL; Department of Biology, Haverford College, Haverford, PA, USA.
  • Charkoudian LK; Department of Chemistry, Haverford College, Haverford, PA, USA.
Microb Genom ; 9(3)2023 03.
Article em En | MEDLINE | ID: mdl-36951894
Nature serves as a rich source of molecules with immense chemical diversity. Aptly named, these 'natural products' boast a wide variety of environmental, medicinal and industrial applications. Type II polyketides, in particular, confer substantial medicinal benefits, including antibacterial, antifungal, anticancer and anti-inflammatory properties. These molecules are produced by enzyme assemblies known as type II polyketide synthases (PKSs), which use domains such as the ketosynthase chain-length factor and acyl carrier protein to produce polyketides with varying lengths, cyclization patterns and oxidation states. In this work, we use a novel bioinformatic workflow to identify biosynthetic gene clusters (BGCs) that code for the core type II PKS enzymes. This method does not rely on annotation and thus was able to unearth previously 'hidden' type II PKS BGCs. This work led us to identify over 6000 putative type II PKS BGCs spanning a diverse set of microbial phyla, nearly double those found in most recent studies. Notably, many of these newly identified BGCs were found in non-actinobacteria, which are relatively underexplored as sources of type II polyketides. Results from this work lay an important foundation for future bioprospecting and engineering efforts that will enable sustainable access to diverse and structurally complex molecules with medicinally relevant properties.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Policetídeo Sintases / Policetídeos Tipo de estudo: Prognostic_studies Idioma: En Revista: Microb Genom Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Policetídeo Sintases / Policetídeos Tipo de estudo: Prognostic_studies Idioma: En Revista: Microb Genom Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos