Retro-orbital CD45 antibody labeling to evaluate antibody-secreting cell trafficking in mice.
STAR Protoc
; 4(2): 102308, 2023 May 12.
Article
em En
| MEDLINE
| ID: mdl-37178113
ABSTRACT
Antibody-secreting cells (ASCs) are critical regulators of the humoral immune response. However, differences between tissue resident populations versus those that have recently migrated to their final anatomic destination are poorly understood. Here, we present a protocol for using retro-orbital (r.o.) CD45 antibody labeling to identify tissue resident versus recently immigrated ASCs in mice. We describe steps for r.o. injection of antibodies, animal euthanasia, and tissue harvesting. We then detail tissue processing, cell counting, and cell staining for flow cytometry analysis. For complete details on the use and execution of this protocol, please refer to Pioli et al. (2023).1.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2023
Tipo de documento:
Article