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Isolation and characterization of the gene HvFAR1 encoding acyl-CoA reductase from the cer-za.227 mutant of barley (Hordeum vulgare) and analysis of the cuticular barrier functions.
Müller, Yannic; Patwari, Payal; Stöcker, Tyll; Zeisler-Diehl, Viktoria; Steiner, Ulrike; Campoli, Chiara; Grewe, Lea; Kuczkowska, Magdalena; Dierig, Maya Marita; Jose, Sarah; Hetherington, Alistair M; Acosta, Ivan F; Schoof, Heiko; Schreiber, Lukas; Dörmann, Peter.
Afiliação
  • Müller Y; Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115, Bonn, Germany.
  • Patwari P; Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115, Bonn, Germany.
  • Stöcker T; Institute of Crop Science and Resource Conservation, University of Bonn, 53115, Bonn, Germany.
  • Zeisler-Diehl V; Institute for Cellular and Molecular Botany, University of Bonn, 53115, Bonn, Germany.
  • Steiner U; Institute of Crop Science and Resource Conservation, University of Bonn, 53115, Bonn, Germany.
  • Campoli C; Division of Plant Sciences, School of Life Sciences, University of Dundee, Dundee, DD2 5DA, UK.
  • Grewe L; James Hutton Institute, Dundee, DD2 5DA, UK.
  • Kuczkowska M; Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115, Bonn, Germany.
  • Dierig MM; Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115, Bonn, Germany.
  • Jose S; Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115, Bonn, Germany.
  • Hetherington AM; School of Biological Sciences, University of Bristol, Bristol, BS8 1TQ, UK.
  • Acosta IF; School of Biological Sciences, University of Bristol, Bristol, BS8 1TQ, UK.
  • Schoof H; Max-Planck Institute for Plant Breeding Research, 50829, Cologne, Germany.
  • Schreiber L; Institute of Crop Science and Resource Conservation, University of Bonn, 53115, Bonn, Germany.
  • Dörmann P; Institute for Cellular and Molecular Botany, University of Bonn, 53115, Bonn, Germany.
New Phytol ; 239(5): 1903-1918, 2023 09.
Article em En | MEDLINE | ID: mdl-37349864
The cuticle is a protective layer covering aerial plant organs. We studied the function of waxes for the establishment of the cuticular barrier in barley (Hordeum vulgare). The barley eceriferum mutants cer-za.227 and cer-ye.267 display reduced wax loads, but the genes affected, and the consequences of the wax changes for the barrier function remained unknown. Cuticular waxes and permeabilities were measured in cer-za.227 and cer-ye.267. The mutant loci were isolated by bulked segregant RNA sequencing. New cer-za alleles were generated by genome editing. The CER-ZA protein was characterized after expression in yeast and Arabidopsis cer4-3. Cer-za.227 carries a mutation in HORVU5Hr1G089230 encoding acyl-CoA reductase (FAR1). The cer-ye.267 mutation is located to HORVU4Hr1G063420 encoding ß-ketoacyl-CoA synthase (KAS1) and is allelic to cer-zh.54. The amounts of intracuticular waxes were strongly decreased in cer-ye.267. The cuticular water loss and permeability of cer-za.227 were similar to wild-type (WT), but were increased in cer-ye.267. Removal of epicuticular waxes revealed that intracuticular, but not epicuticular waxes are required to regulate cuticular transpiration. The differential decrease in intracuticular waxes between cer-za.227 and cer-ye.267, and the removal of epicuticular waxes indicate that the cuticular barrier function mostly depends on the presence of intracuticular waxes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hordeum / Arabidopsis / Proteínas de Saccharomyces cerevisiae / Proteínas de Arabidopsis Idioma: En Revista: New Phytol Assunto da revista: BOTANICA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hordeum / Arabidopsis / Proteínas de Saccharomyces cerevisiae / Proteínas de Arabidopsis Idioma: En Revista: New Phytol Assunto da revista: BOTANICA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Alemanha