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Metabolomic profile of seminal plasma from Guzerá bulls (Bos indicus) with contrasting sperm freezability phenotypes.
Rodrigues Pessoa, Eduardo; Roger Vasconcelos, Fábio; de Oliveira Paula-Marinho, Stelamaris; de Menezes Daloso, Danilo; Damasceno Guerreiro, Denise; Matias Martins, Jorge André; Gomes-Filho, Enéas; Alencar Moura, Arlindo.
Afiliação
  • Rodrigues Pessoa E; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.
  • Roger Vasconcelos F; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.
  • de Oliveira Paula-Marinho S; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Brazil.
  • de Menezes Daloso D; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Brazil.
  • Damasceno Guerreiro D; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.
  • Matias Martins JA; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.
  • Gomes-Filho E; Center for Agricultural Sciences and Biodiversity, Federal University of Cariri, Crato, Brazil.
  • Alencar Moura A; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Brazil.
Reprod Domest Anim ; 58(10): 1379-1392, 2023 Oct.
Article em En | MEDLINE | ID: mdl-37592767
The present study evaluated the seminal plasma metabolome of Bos indicus Guzerá bulls with good (n = 4) and poor (n = 5) sperm freezability. Animals were raised in natural pasture of a 'Caatinga' ecosystem, in the semi-arid region of Brazil. Seminal plasma samples were subjected to gas chromatography coupled to mass spectrometry and data, analysed using bioinformatics tools (Cytoscape with the MetScape plug-in). Sixty-two metabolites were identified in the bovine seminal plasma. Fatty acids and conjugates and organic compounds were the predominant seminal fluid metabolites, followed by carboxylic acids and derivatives, amino acids, benzenes and steroids and derivatives, carbohydrates and carbohydrate conjugates and prenol lipids. Multivariate analysis indicated a distinct separation of seminal plasma metabolomes from bulls with contrasting sperm freezability. Abundances of propanoic acid, d-ribose and glycine were greater in the seminal plasma of bulls with good sperm freezability. Heptadecanoic acid and undecanoic acid were the predominant in bulls of poor sperm freezability. Propanoic acid is an energy source for spermatozoa and may act as an antimicrobial component in semen. Glycine acts against oxidizing and denaturing reactions. d-ribose is also an energy source and reduces apoptosis and oxidative stress. Undecanoic acid may protect sperm against fungal damage. This study provides fundamental information approximately the seminal plasma metabolome of tropically adapted bulls and its association with sperm freezability. However, further studies with larger groups of animals are needed to validate those metabolites as markers of sperm freezability. This strategy could support the selection of sires with superior sperm cryoresistance.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Propionatos / Sêmen Limite: Animals Idioma: En Revista: Reprod Domest Anim Assunto da revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Propionatos / Sêmen Limite: Animals Idioma: En Revista: Reprod Domest Anim Assunto da revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil