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The microenvironment dictates glycocalyx construction and immune surveillance.
Tharp, Kevin M; Park, Sangwoo; Timblin, Greg A; Richards, Alicia L; Berg, Jordan A; Twells, Nicholas M; Riley, Nicholas M; Peltan, Egan L; Shon, D Judy; Stevenson, Erica; Tsui, Kimberly; Palomba, Francesco; Lefebvre, Austin E Y T; Soens, Ross W; Ayad, Nadia M E; Hoeve-Scott, Johanna Ten; Healy, Kevin; Digman, Michelle; Dillin, Andrew; Bertozzi, Carolyn R; Swaney, Danielle L; Mahal, Lara K; Cantor, Jason R; Paszek, Matthew J; Weaver, Valerie M.
Afiliação
  • Tharp KM; Center for Bioengineering and Tissue Regeneration, Department of Surgery, University of California San Francisco, San Francisco, CA 94143, USA.
  • Park S; Field of Biophysics, Cornell University, Ithaca, NY 14850, USA.
  • Timblin GA; Center for Bioengineering and Tissue Regeneration, Department of Surgery, University of California San Francisco, San Francisco, CA 94143, USA.
  • Richards AL; Quantitative Biosciences Institute (QBI) and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA; J. David Gladstone Institutes, San Francisco, CA 94158, USA.
  • Berg JA; Department of Biochemistry, University of Utah, Salt Lake City, UT 84112, USA.
  • Twells NM; Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada.
  • Riley NM; Department of Chemistry, Sarafan ChEM-H, Stanford University, Stanford, CA, USA.
  • Peltan EL; Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford CA USA 94305.
  • Shon DJ; Sarafan ChEM-H, Stanford University, Stanford, CA USA 94305.
  • Stevenson E; Department of Chemistry, Sarafan ChEM-H, Stanford University, Stanford, CA, USA.
  • Tsui K; Quantitative Biosciences Institute (QBI) and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA; J. David Gladstone Institutes, San Francisco, CA 94158, USA.
  • Palomba F; Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94597, USA.
  • Lefebvre AEYT; Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, CA 92697, USA.
  • Soens RW; Calico Life Sciences, South San Francisco, CA, USA.
  • Ayad NME; Morgridge Institute for Research, Madison, WI 53715, USA; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
  • Hoeve-Scott JT; Center for Bioengineering and Tissue Regeneration, Department of Surgery, University of California San Francisco, San Francisco, CA 94143, USA.
  • Healy K; UCLA Metabolomics Center, Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
  • Digman M; Department of Chemical and Systems Biology, Sarafan ChEM-H and Howard Hughes Medical Institute, Stanford University, Stanford, CA USA 94305.
  • Dillin A; Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, CA 92697, USA.
  • Bertozzi CR; Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94597, USA.
  • Swaney DL; Department of Chemical and Systems Biology, Sarafan ChEM-H and Howard Hughes Medical Institute, Stanford University, Stanford, CA USA 94305.
  • Mahal LK; Quantitative Biosciences Institute (QBI) and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA; J. David Gladstone Institutes, San Francisco, CA 94158, USA.
  • Cantor JR; Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada.
  • Paszek MJ; Morgridge Institute for Research, Madison, WI 53715, USA; Department of Biochemistry and Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53706, USA.
  • Weaver VM; Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14850, USA.
Res Sq ; 2023 Aug 16.
Article em En | MEDLINE | ID: mdl-37645943
ABSTRACT
Efforts to identify anti-cancer therapeutics and understand tumor-immune interactions are built with in vitro models that do not match the microenvironmental characteristics of human tissues. Using in vitro models which mimic the physical properties of healthy or cancerous tissues and a physiologically relevant culture medium, we demonstrate that the chemical and physical properties of the microenvironment regulate the composition and topology of the glycocalyx. Remarkably, we find that cancer and age-related changes in the physical properties of the microenvironment are sufficient to adjust immune surveillance via the topology of the glycocalyx, a previously unknown phenomenon observable only with a physiologically relevant culture medium.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies / Screening_studies Idioma: En Revista: Res Sq Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies / Screening_studies Idioma: En Revista: Res Sq Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos