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A maternally programmed intergenerational mechanism enables male offspring to make piRNAs from Y-linked precursor RNAs in Drosophila.
Venkei, Zsolt G; Gainetdinov, Ildar; Bagci, Ayca; Starostik, Margaret R; Choi, Charlotte P; Fingerhut, Jaclyn M; Chen, Peiwei; Balsara, Chiraag; Whitfield, Troy W; Bell, George W; Feng, Suhua; Jacobsen, Steven E; Aravin, Alexei A; Kim, John K; Zamore, Phillip D; Yamashita, Yukiko M.
Afiliação
  • Venkei ZG; Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Gainetdinov I; RNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA, USA.
  • Bagci A; RNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA, USA.
  • Starostik MR; Department of Biology, Johns Hopkins University, Baltimore, MD, USA.
  • Choi CP; Department of Biology, Johns Hopkins University, Baltimore, MD, USA.
  • Fingerhut JM; Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Chen P; Howard Hughes Medical Institute, Whitehead Institute, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Balsara C; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
  • Whitfield TW; Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.
  • Bell GW; Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Feng S; Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Jacobsen SE; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA.
  • Aravin AA; Eli and Edyth Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA.
  • Kim JK; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA.
  • Zamore PD; Eli and Edyth Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA.
  • Yamashita YM; Howard Hughes Medical Institute, University of California Los Angeles, Los Angeles, CA, USA.
Nat Cell Biol ; 25(10): 1495-1505, 2023 Oct.
Article em En | MEDLINE | ID: mdl-37723298
ABSTRACT
In animals, PIWI-interacting RNAs (piRNAs) direct PIWI proteins to silence complementary targets such as transposons. In Drosophila and other species with a maternally specified germline, piRNAs deposited in the egg initiate piRNA biogenesis in the progeny. However, Y chromosome loci cannot participate in such a chain of intergenerational inheritance. How then can the biogenesis of Y-linked piRNAs be initiated? Here, using Suppressor of Stellate (Su(Ste)), a Y-linked Drosophila melanogaster piRNA locus as a model, we show that Su(Ste) piRNAs are made in the early male germline via 5'-to-3' phased piRNA biogenesis initiated by maternally deposited 1360/Hoppel transposon piRNAs. Notably, deposition of Su(Ste) piRNAs from XXY mothers obviates the need for phased piRNA biogenesis in sons. Together, our study uncovers a developmentally programmed, intergenerational mechanism that allows fly mothers to protect their sons using a Y-linked piRNA locus.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Drosophila / Drosophila Limite: Animals Idioma: En Revista: Nat Cell Biol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Drosophila / Drosophila Limite: Animals Idioma: En Revista: Nat Cell Biol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos