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Impact of tectoridin on the pharmacokinetics of florfenicol via targeting cytochrome P450 and P-glycoprotein of rats.
Zhang, Min; Wang, Bin; Li, Xu-Ting; Yin, Qin; Liang, Ge; Li, Si-Cong.
Afiliação
  • Zhang M; Sichuan Key Laboratory of Animal Genetics and Breeding, Sichuan Animal Science Academy, Chengdu, China.
  • Wang B; Veterinary Natural Medicine Research and GCP Experimental Animal Centre, Lezhi, China.
  • Li XT; Sichuan Key Laboratory of Animal Genetics and Breeding, Sichuan Animal Science Academy, Chengdu, China.
  • Yin Q; Veterinary Natural Medicine Research and GCP Experimental Animal Centre, Lezhi, China.
  • Liang G; Sichuan Key Laboratory of Animal Genetics and Breeding, Sichuan Animal Science Academy, Chengdu, China.
  • Li SC; Veterinary Natural Medicine Research and GCP Experimental Animal Centre, Lezhi, China.
Xenobiotica ; 53(5): 429-437, 2023 May.
Article em En | MEDLINE | ID: mdl-37781957
ABSTRACT
Belamcanda chinensis (L.) DC, commonly used with florfenicol in Chinese veterinary clinics for respiratory tract infections, contains the major effective isoflavone, tectoridin (TEC). This study aimed to investigate the impact of TEC co-administration on the pharmacokinetics of florfenicol in vivo.Male rats received oral TEC (50 mg/kg BW) or sterile water for seven days, followed by a single oral dose of florfenicol (25 mg/kg BW) on the 8th day. Non-compartmental methods analysed the pharmacokinetics of florfenicol, while real-time reverse transcription polymerase chain reaction (RT-PCR), Western blot, and immunohistochemical analyses measured expression levels of cytochrome P450 (CYP) isoforms in the liver and P-glycoprotein (P-gp) in the jejunum.TEC significantly decreased florfenicol's AUC(0-∞), MRT(0-∞), t1/2z, Vz/F, and Cmax by 24.75%, 18.43%, 55.47%, 43.05%, and 19.48%, while increasing CLz/F by 33.33%. TEC also up-regulated hepatic CYP1A2 and CYP3A1 mRNA expression, as well as intestinal MDR1, by 1.39-fold, 1.85-fold, and 1.65-fold. This coincided with a respective increase in protein expression by 1.37-fold, 1.39-fold, and 1.43-fold.These findings suggest that TEC-induced alterations in the pharmacokinetics of florfenicol may be attributed to increased CYP and P-gp expression. Further investigations are warranted to understand the implications of these findings on the clinical effectiveness of florfenicol in veterinary practice.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Membro 1 da Subfamília B de Cassetes de Ligação de ATP / Isoflavonas Limite: Animals Idioma: En Revista: Xenobiotica Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Membro 1 da Subfamília B de Cassetes de Ligação de ATP / Isoflavonas Limite: Animals Idioma: En Revista: Xenobiotica Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China