Single-cell RNA sequencing of bronchoscopy specimens: development of a rapid minimal handling protocol.
Biotechniques
; 75(4): 157-167, 2023 10.
Article
em En
| MEDLINE
| ID: mdl-37815826
ABSTRACT
Single-cell RNA sequencing (scRNA-seq) is an important tool for understanding disease pathophysiology, including airway diseases. Currently, the majority of scRNA-seq studies in airway diseases have used invasive methods (airway biopsy, surgical resection), which carry inherent risks and thus present a major limitation to scRNA-seq investigation of airway pathobiology. Bronchial brushing, where the airway mucosa is sampled using a cytological brush, is a viable, less invasive method of obtaining airway cells for scRNA-seq. Here we describe the development of a rapid and minimal handling protocol for preparing single-cell suspensions from bronchial brush specimens for scRNA-seq. Our optimized protocol maximizes cell recovery and cell quality and facilitates large-scale profiling of the airway transcriptome at single-cell resolution.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Software
/
Perfilação da Expressão Gênica
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Canadá