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Circulating cell-free DNA fragmentation is a stepwise and conserved process linked to apoptosis.
Zhu, Dandan; Wang, Haihong; Wu, Wei; Geng, Shuaipeng; Zhong, Guolin; Li, Yunfei; Guo, Han; Long, Guanghui; Ren, Qingqi; Luan, Yi; Duan, Chaohui; Wei, Bing; Ma, Jie; Li, Shiyong; Zhou, Jun; Mao, Mao.
Afiliação
  • Zhu D; Clinical Laboratories, Shenyou Bio, Zhengzhou, 450000, China.
  • Wang H; Shanghai Institute of Hematology, CNRS-LIA Hematology and Cancer, Sino-French Research Center for Life Sciences and Genomics, State Key Laboratory of Medical Genomics, Rui Jin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
  • Wu W; Research & Development, SeekIn Inc, Shenzhen, 518000, China.
  • Geng S; Clinical Laboratories, Shenyou Bio, Zhengzhou, 450000, China.
  • Zhong G; Research & Development, SeekIn Inc, Shenzhen, 518000, China.
  • Li Y; Research & Development, SeekIn Inc, Shenzhen, 518000, China.
  • Guo H; Clinical Laboratories, Shenyou Bio, Zhengzhou, 450000, China.
  • Long G; Department of Hepatobiliary and Pancreatic Surgery, Peking University Shenzhen Hospital, Shenzhen, 518000, China.
  • Ren Q; Department of Hepatobiliary and Pancreatic Surgery, Peking University Shenzhen Hospital, Shenzhen, 518000, China.
  • Luan Y; Clinical Laboratories, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510000, China.
  • Duan C; School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
  • Wei B; Clinical Laboratories, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510000, China.
  • Ma J; Department of Molecular Pathology, The Affiliated Cancer Hospital of Zhengzhou University and Henan Cancer Hospital, Zhengzhou, 450003, China.
  • Li S; Department of Molecular Pathology, The Affiliated Cancer Hospital of Zhengzhou University and Henan Cancer Hospital, Zhengzhou, 450003, China.
  • Zhou J; Research & Development, SeekIn Inc, Shenzhen, 518000, China.
  • Mao M; Shanghai Institute of Hematology, CNRS-LIA Hematology and Cancer, Sino-French Research Center for Life Sciences and Genomics, State Key Laboratory of Medical Genomics, Rui Jin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China. zj10802@rjh.com.cn.
BMC Biol ; 21(1): 253, 2023 11 13.
Article em En | MEDLINE | ID: mdl-37953260
BACKGROUND: Circulating cell-free DNA (cfDNA) is a pool of short DNA fragments mainly released from apoptotic hematopoietic cells. Nevertheless, the precise physiological process governing the DNA fragmentation and molecular profile of cfDNA remains obscure. To dissect the DNA fragmentation process, we use a human leukemia cell line HL60 undergoing apoptosis to analyze the size distribution of DNA fragments by shallow whole-genome sequencing (sWGS). Meanwhile, we also scrutinize the size profile of plasma cfDNA in 901 healthy human subjects and 38 dogs, as well as 438 patients with six common cancer types by sWGS. RESULTS: Distinct size distribution profiles were observed in the HL60 cell pellet and supernatant, suggesting fragmentation is a stepwise process. Meanwhile, C-end preference was seen in both intracellular and extracellular cfDNA fragments. Moreover, the cfDNA profiles are characteristic and conserved across mammals. Compared with healthy subjects, distinct cfDNA profiles with a higher proportion of short fragments and lower C-end preference were found in cancer patients. CONCLUSIONS: Our study provides new insight into fragmentomics of circulating cfDNA processing, which will be useful for early diagnosis of cancer and surveillance during cancer progression.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácidos Nucleicos Livres / Neoplasias Limite: Animals / Humans Idioma: En Revista: BMC Biol Assunto da revista: BIOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácidos Nucleicos Livres / Neoplasias Limite: Animals / Humans Idioma: En Revista: BMC Biol Assunto da revista: BIOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China