Molecular Basis for SPINDOC-Spindlin1 Engagement and Its Role in Transcriptional Attenuation.
J Mol Biol
; 436(7): 168371, 2024 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-37977297
ABSTRACT
Spindlin1 is a histone reader with three Tudor-like domains and its transcriptional co-activator activity could be attenuated by SPINDOC. The first two Tudors are involved in histone methylation readout, while the function of Tudor 3 is largely unknown. Here our structural and binding studies revealed an engagement mode of SPINDOC-Spindlin1, in which a hydrophobic motif of SPINDOC, DOCpep3, stably interacts with Spindlin1 Tudor 3, and two neighboring K/R-rich motifs, DOCpep1 and DOCpep2, bind to the acidic surface of Spindlin1 Tudor 2. Although DOCpep3-Spindlin1 engagement is compatible with histone readout, an extended SPINDOC fragment containing the K/R-rich region attenuates histone or TCF4 binding by Spindlin1 due to introduced competition. This inhibitory effect is more pronounced for weaker binding targets but not for strong ones such as H3 "K4me3-K9me3" bivalent mark. Further ChIP-seq and RT-qPCR indicated that SPINDOC could promote genomic relocation of Spindlin1, thus modulate downstream gene transcription. Collectively, we revealed multivalent engagement between SPINDOC and Spindlin1, in which a hydrophobic motif acts as the primary binding site for stable SPINDOC-Spindlin1 association, while K/R-rich region modulates the target selectivity of Spindlin1 via competitive inhibition, therefore attenuating the transcriptional co-activator activity of Spindlin1.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
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Transcrição Gênica
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Histonas
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Regulação da Expressão Gênica
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Proteínas de Ciclo Celular
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Domínios e Motivos de Interação entre Proteínas
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Proteínas Correpressoras
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Domínio Tudor
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Proteínas Associadas aos Microtúbulos
Limite:
Humans
Idioma:
En
Revista:
J Mol Biol
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China