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Visualizing the DNA repair process by a photolyase at atomic resolution.
Maestre-Reyna, Manuel; Wang, Po-Hsun; Nango, Eriko; Hosokawa, Yuhei; Saft, Martin; Furrer, Antonia; Yang, Cheng-Han; Gusti Ngurah Putu, Eka Putra; Wu, Wen-Jin; Emmerich, Hans-Joachim; Caramello, Nicolas; Franz-Badur, Sophie; Yang, Chao; Engilberge, Sylvain; Wranik, Maximilian; Glover, Hannah Louise; Weinert, Tobias; Wu, Hsiang-Yi; Lee, Cheng-Chung; Huang, Wei-Cheng; Huang, Kai-Fa; Chang, Yao-Kai; Liao, Jiahn-Haur; Weng, Jui-Hung; Gad, Wael; Chang, Chiung-Wen; Pang, Allan H; Yang, Kai-Chun; Lin, Wei-Ting; Chang, Yu-Chen; Gashi, Dardan; Beale, Emma; Ozerov, Dmitry; Nass, Karol; Knopp, Gregor; Johnson, Philip J M; Cirelli, Claudio; Milne, Chris; Bacellar, Camila; Sugahara, Michihiro; Owada, Shigeki; Joti, Yasumasa; Yamashita, Ayumi; Tanaka, Rie; Tanaka, Tomoyuki; Luo, Fangjia; Tono, Kensuke; Zarzycka, Wiktoria; Müller, Pavel; Alahmad, Maisa Alkheder.
Afiliação
  • Maestre-Reyna M; Institute of Biological Chemistry, Academia Sinica, 128 Academia Rd. Sec. 2, Nankang, Taipei 115, Taiwan.
  • Wang PH; Department of Chemistry, National Taiwan University, 1, Roosevelt Rd. Sec. 4, Taipei 106, Taiwan.
  • Nango E; Institute of Biological Chemistry, Academia Sinica, 128 Academia Rd. Sec. 2, Nankang, Taipei 115, Taiwan.
  • Hosokawa Y; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
  • Saft M; Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan.
  • Furrer A; Institute of Biological Chemistry, Academia Sinica, 128 Academia Rd. Sec. 2, Nankang, Taipei 115, Taiwan.
  • Yang CH; Department of Chemistry, National Taiwan University, 1, Roosevelt Rd. Sec. 4, Taipei 106, Taiwan.
  • Gusti Ngurah Putu EP; Division of Chemistry, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.
  • Wu WJ; Department of Chemistry, Philipps University Marburg, Hans-Meerwein Strasse 4, Marburg 35032, Germany.
  • Emmerich HJ; Paul Scherrer Institute, Forschungstrasse 111, 5232 Villigen PSI, Switzerland.
  • Caramello N; Institute of Biological Chemistry, Academia Sinica, 128 Academia Rd. Sec. 2, Nankang, Taipei 115, Taiwan.
  • Franz-Badur S; Institute of Biological Chemistry, Academia Sinica, 128 Academia Rd. Sec. 2, Nankang, Taipei 115, Taiwan.
  • Yang C; Institute of Biological Chemistry, Academia Sinica, 128 Academia Rd. Sec. 2, Nankang, Taipei 115, Taiwan.
  • Engilberge S; Department of Chemistry, Philipps University Marburg, Hans-Meerwein Strasse 4, Marburg 35032, Germany.
  • Wranik M; European Synchrotron Radiation Facility, 38043 Grenoble, France.
  • Glover HL; Hamburg Centre for Ultrafast Imaging, Universität Hamburg, 22761 Hamburg, Germany.
  • Weinert T; Department of Chemistry, Philipps University Marburg, Hans-Meerwein Strasse 4, Marburg 35032, Germany.
  • Wu HY; Department of Physics, The Ohio State University, Columbus, OH 43210, USA.
  • Lee CC; European Synchrotron Radiation Facility, 38043 Grenoble, France.
  • Huang WC; Université Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale (IBS), 38044 Grenoble, France.
  • Huang KF; Paul Scherrer Institute, Forschungstrasse 111, 5232 Villigen PSI, Switzerland.
  • Chang YK; Paul Scherrer Institute, Forschungstrasse 111, 5232 Villigen PSI, Switzerland.
  • Liao JH; Paul Scherrer Institute, Forschungstrasse 111, 5232 Villigen PSI, Switzerland.
  • Weng JH; Institute of Biological Chemistry, Academia Sinica, 128 Academia Rd. Sec. 2, Nankang, Taipei 115, Taiwan.
  • Gad W; Institute of Biological Chemistry, Academia Sinica, 128 Academia Rd. Sec. 2, Nankang, Taipei 115, Taiwan.
  • Chang CW; Institute of Biological Chemistry, Academia Sinica, 128 Academia Rd. Sec. 2, Nankang, Taipei 115, Taiwan.
  • Pang AH; Institute of Biological Chemistry, Academia Sinica, 128 Academia Rd. Sec. 2, Nankang, Taipei 115, Taiwan.
  • Yang KC; Institute of Biological Chemistry, Academia Sinica, 128 Academia Rd. Sec. 2, Nankang, Taipei 115, Taiwan.
  • Lin WT; Institute of Biological Chemistry, Academia Sinica, 128 Academia Rd. Sec. 2, Nankang, Taipei 115, Taiwan.
  • Chang YC; Institute of Biological Chemistry, Academia Sinica, 128 Academia Rd. Sec. 2, Nankang, Taipei 115, Taiwan.
  • Gashi D; Institute of Biological Chemistry, Academia Sinica, 128 Academia Rd. Sec. 2, Nankang, Taipei 115, Taiwan.
  • Beale E; Institute of Biological Chemistry, Academia Sinica, 128 Academia Rd. Sec. 2, Nankang, Taipei 115, Taiwan.
  • Ozerov D; Institute of Biological Chemistry, Academia Sinica, 128 Academia Rd. Sec. 2, Nankang, Taipei 115, Taiwan.
  • Nass K; Department of Chemistry, National Taiwan University, 1, Roosevelt Rd. Sec. 4, Taipei 106, Taiwan.
  • Knopp G; Department of Chemistry, National Taiwan University, 1, Roosevelt Rd. Sec. 4, Taipei 106, Taiwan.
  • Johnson PJM; Department of Chemistry, National Taiwan University, 1, Roosevelt Rd. Sec. 4, Taipei 106, Taiwan.
  • Cirelli C; Paul Scherrer Institute, Forschungstrasse 111, 5232 Villigen PSI, Switzerland.
  • Milne C; Paul Scherrer Institute, Forschungstrasse 111, 5232 Villigen PSI, Switzerland.
  • Bacellar C; Paul Scherrer Institute, Forschungstrasse 111, 5232 Villigen PSI, Switzerland.
  • Sugahara M; Paul Scherrer Institute, Forschungstrasse 111, 5232 Villigen PSI, Switzerland.
  • Owada S; Paul Scherrer Institute, Forschungstrasse 111, 5232 Villigen PSI, Switzerland.
  • Joti Y; Paul Scherrer Institute, Forschungstrasse 111, 5232 Villigen PSI, Switzerland.
  • Yamashita A; Paul Scherrer Institute, Forschungstrasse 111, 5232 Villigen PSI, Switzerland.
  • Tanaka R; Paul Scherrer Institute, Forschungstrasse 111, 5232 Villigen PSI, Switzerland.
  • Tanaka T; Paul Scherrer Institute, Forschungstrasse 111, 5232 Villigen PSI, Switzerland.
  • Luo F; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
  • Tono K; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
  • Zarzycka W; Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5198, Japan.
  • Müller P; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
  • Alahmad MA; Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5198, Japan.
Science ; 382(6674): eadd7795, 2023 12.
Article em En | MEDLINE | ID: mdl-38033054
ABSTRACT
Photolyases, a ubiquitous class of flavoproteins, use blue light to repair DNA photolesions. In this work, we determined the structural mechanism of the photolyase-catalyzed repair of a cyclobutane pyrimidine dimer (CPD) lesion using time-resolved serial femtosecond crystallography (TR-SFX). We obtained 18 snapshots that show time-dependent changes in four reaction loci. We used these results to create a movie that depicts the repair of CPD lesions in the picosecond-to-nanosecond range, followed by the recovery of the enzymatic moieties involved in catalysis, completing the formation of the fully reduced enzyme-product complex at 500 nanoseconds. Finally, back-flip intermediates of the thymine bases to reanneal the DNA were captured at 25 to 200 microseconds. Our data cover the complete molecular mechanism of a photolyase and, importantly, its chemistry and enzymatic catalysis at work across a wide timescale and at atomic resolution.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dímeros de Pirimidina / Methanosarcina / Proteínas Arqueais / Desoxirribodipirimidina Fotoliase / Reparo do DNA Idioma: En Revista: Science Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Taiwan
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dímeros de Pirimidina / Methanosarcina / Proteínas Arqueais / Desoxirribodipirimidina Fotoliase / Reparo do DNA Idioma: En Revista: Science Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Taiwan