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Interaction between membrane curvature sensitive factors SpoVM and SpoIVA in Bicelle condition.
Chen, Jiali; Wang, Yifan; Lin, Shuru; Yu, Quanxiang; Qi, Zhengfei; Jiang, Wenqi; Zhao, Qiang; Fu, Qingshan Bill.
Afiliação
  • Chen J; School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China; Shanghai Institute of Materia Medica, Zhongshan Institute for Drug Discovery, Chinese Academy of Sciences, Zhongshan, 528400, China.
  • Wang Y; Shanghai Institute of Materia Medica, Zhongshan Institute for Drug Discovery, Chinese Academy of Sciences, Zhongshan, 528400, China.
  • Lin S; Shanghai Institute of Materia Medica, Zhongshan Institute for Drug Discovery, Chinese Academy of Sciences, Zhongshan, 528400, China.
  • Yu Q; Shanghai Institute of Materia Medica, Zhongshan Institute for Drug Discovery, Chinese Academy of Sciences, Zhongshan, 528400, China.
  • Qi Z; Shanghai Institute of Materia Medica, Zhongshan Institute for Drug Discovery, Chinese Academy of Sciences, Zhongshan, 528400, China.
  • Jiang W; Shanghai Institute of Materia Medica, Zhongshan Institute for Drug Discovery, Chinese Academy of Sciences, Zhongshan, 528400, China.
  • Zhao Q; School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China; Shanghai Institute of Materia Medica, Zhongshan Institute for Drug Discovery, Chinese Academy of Sciences, Zhongshan, 528400, China. Electronic address: zhaoq@simm.ac.cn.
  • Fu QB; School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China; Shanghai Institute of Materia Medica, Zhongshan Institute for Drug Discovery, Chinese Academy of Sciences, Zhongshan, 528400, China. Electronic address: fuqingshan@simm.ac.cn.
Biochem Biophys Res Commun ; 694: 149395, 2024 Jan 29.
Article em En | MEDLINE | ID: mdl-38141557
ABSTRACT
SpoVM and SpoIVA are essential proteins for coat assembly in Bacillus subtilis. SpoVM is a membrane curvature sensor, specifically localized on the forespore membrane. SpoIVA is an ATP hydrolase that self-assembles by hydrolyzing ATP. In this work, SpoVM and its mutant SpoVMP9A were obtained by cyanogen bromide cleavage and reconstituted into bicelles. The purification of SpoIVA was achieved through a rigorous process involving Ni-NTA chromatography column and size exclusion chromatography. This study utilized Biacore to obtain a direct determination of the kinetic parameters of interaction between SpoVM (SpoVMP9A) and SpoIVA in Bicelle conditions.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Trifosfato de Adenosina Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Trifosfato de Adenosina Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China