Salinity and exogenous H2 O2 improve gas exchange, osmoregulation, and antioxidant metabolism in quinoa under drought stress.

ABSTRACT

Climate change-induced concurrent drought and salinity stresses significantly threaten global crop yields, yet the physio-biochemical responses to combined stress in quinoa remain elusive. This study evaluated quinoa responses under four growth conditions well-watered, drought stress, salt stress, and drought + salt stress with (15 mM) or without (0 mM) exogenous hydrogen peroxide (H2 O2 ) application. All examined stresses (alone or in combination) reduce quinoa growth and net photosynthesis, although salt stress was found to be less destructive than drought and combined stress. Strikingly, superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), stomatal conductance (gs ), photosynthetic rate (PN ), K+ uptake, shoot height, shoot fresh, and dry weight were increased by 46.1%, 22.2%, 101.6%, 12.9%, 12.1%, 22.4%, 7.1%, 14%, and 16.4%, respectively, under combined stress compared to drought alone. In addition, exogenous H2 O2 effectively improved gaseous exchange, osmolytes' accumulation, and antioxidant activity, resulting in reduced lipid peroxidation, which eventually led to higher plant growth under all coercive conditions. The principle component analysis (PCA) indicated a strong positive correlation between antioxidant enzymes and inorganic ions, which contributed efficiently to osmotic adjustment, particularly under conditions of salinity followed by combined stress. In short, in combination, salt stress has the potential to mitigate drought-induced injuries by promoting the absorption of inorganic solutes for osmoregulation in quinoa plants. Furthermore, exogenous application of H2 O2 could be opted to enhance quinoa performance to increase its tolerance mechanism against drought and salinity, even under combined stress.