Headplate Installation and Craniotomy for Awake In Vivo Electrophysiological Recordings or Two-Photon Imaging of the Mouse Inferior Colliculus.
Bio Protoc
; 13(24): e4902, 2023 Dec 20.
Article
em En
| MEDLINE
| ID: mdl-38156034
ABSTRACT
The inferior colliculus (IC) is an important processing center in the auditory system, which also receives non-auditory sensory input. The IC consists of several subnuclei whose functional role in (non-) auditory processing and plastic response properties are best approached by studying awake animals, preferably in a longitudinal fashion. The increasing use of mice in auditory research, the availability of genetic models, and the superficial location of the IC in the mouse have made it an attractive species for studying IC function. Here, we describe a protocol for exposing the mouse IC for up to a few weeks for in vivo imaging or electrophysiology in a stable manner. This method allows for a broader sampling of the IC while maintaining the brain surface in good quality and without reopening the craniotomy. Moreover, as it is adaptable for both electrophysiological recordings of the entire IC and imaging of the dorsal IC surface, it can be applied to answer a multitude of questions. Key features ⢠A surgical protocol for long-term physiological recordings from the same or separate neuronal populations in the inferior colliculus. ⢠Optimized for awake in vivo experiments in the house mouse (Mus musculus).
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Bio Protoc
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Holanda