Split G-quadruplex based PfAgo sensing platform for nucleotide mutation discrimination and human genotyping.

Zhang, Yan; Gong, Bin; Lin, Yanan; Zhu, Yuedong; Su, Gaoxing; Yu, Yanyan

Zhang, Yan; Gong, Bin; Lin, Yanan; Zhu, Yuedong; Su, Gaoxing; Yu, Yanyan.

Afiliação

Zhang Y; School of Pharmacy, Nantong University, Nantong, Jiangsu 226001, China. sugaoxing@ntu.edu.cn.
Gong B; School of Pharmacy, Nantong University, Nantong, Jiangsu 226001, China. sugaoxing@ntu.edu.cn.
Lin Y; School of Pharmacy, Nantong University, Nantong, Jiangsu 226001, China. sugaoxing@ntu.edu.cn.
Zhu Y; School of Pharmacy, Nantong University, Nantong, Jiangsu 226001, China. sugaoxing@ntu.edu.cn.
Su G; School of Pharmacy, Nantong University, Nantong, Jiangsu 226001, China. sugaoxing@ntu.edu.cn.
Yu Y; School of Pharmacy, Nantong University, Nantong, Jiangsu 226001, China. sugaoxing@ntu.edu.cn.

Analyst ; 149(3): 707-711, 2024 Jan 29.

Article em En | MEDLINE | ID: mdl-38230655

ABSTRACT

ABSTRACT

A PfAgo-G4 sensing platform exploiting G4 as a signal reporter was proposed, validated, and optimized. By introducing two mismatches at the Link strand, a universal nucleotide design rule was established for accurate single nucleotide polymorphism discrimination with PfAgo-G4. The FUT2 gene was then successfully and accurately genotyped using human buccal swab samples.

Assuntos

Aptâmeros de Nucleotídeos; Quadruplex G; Humanos; Genótipo; Polimorfismo de Nucleotídeo Único; Aptâmeros de Nucleotídeos/genética

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aptâmeros de Nucleotídeos / Quadruplex G Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Analyst Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

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