Endonuclease IV and T4 ligase enhanced detection of mutations in low abundance.
Analyst
; 149(4): 1050-1054, 2024 Feb 12.
Article
em En
| MEDLINE
| ID: mdl-38231135
ABSTRACT
We propose a mutant detection approach based on endonuclease IV and DNA ligase in combination with qPCR. The enzymes functioned cooperatively to facilitate PCR for low abundance DNA detection. We demonstrate that our approach can distinguish mutations as low as 0.01%, indicating the potential application of this strategy in early cancer diagnosis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Ligases
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Analyst
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China