HPLC-MS/MS Profiles, Antioxidant, Neuroprotective, Antidiabetic and Skin Protective Effects of Different Extracts of Vicia peregrina L. Collected from the Eastern Region of Turkey.
Chem Biodivers
; 21(3): e202400040, 2024 Mar.
Article
em En
| MEDLINE
| ID: mdl-38265183
ABSTRACT
Many Vicia species (Fabaceae) were proven to possess bioactive compounds with potential health beneficial properties. The present study was designed to determine the phenolic constituents, antioxidant and enzyme inhibition activities of aerial parts and seed of V.â
peregrina. Hexane, ethyl acetate and methanol extracts were prepared by maceration and aqueous extract by infusion. The chemical compositions of the extracts were determined using HPLC-MS/MS technology. The antioxidant activities were examined using various assays including free radical scavenging (ABTS and DPPH), reducing ability (CUPRAC and FRAP), metal chelation, and phosphomolybdenum. The enzyme inhibitory effects were investigated against cholinesterase, tyrosinase, amylase and glucosidase. The highest total phenolics and flavonoids contents were recorded in the methanol extracts of the seed (45.42â
mg GAE/g) and aerial parts (40.33â
mg RE/g) respectively. The aerial parts were characterized by higher accumulation of chlorogenic acid (9893.86â
µg g-1 ), isoquercitrin (9400.33â
µg g-1 ), delphindin 3,5 diglucoside (9113.28â
µg g-1 ), hyperoside (6337.09â
µg g-1 ), rutin (3489.83â
µg g-1 ) and kaempferol-3-glucoside (2872.84â
µg g-1 ). Generally, the methanol and aqueous extracts of the two studied parts exerted the best antioxidant activity with highest anti-DPPH (61.99â
mg TE/g), anti-ABTS (101.80â
mg TE/g) and Cu++ (16169â
mg TE/g) and Fe+++ (172,36â
mg TE/g) reducing capacity were recorded from the seed methanol extract. Methanol extract of the seed showed the best anti-tyrosinase activity (75.86â
mg KAE/g). These results indicated that V. peregrina is rich with bioactive phenolics suggesting their use in different health promoting applications.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vicia
/
Antioxidantes
País/Região como assunto:
Asia
Idioma:
En
Revista:
Chem Biodivers
Assunto da revista:
BIOQUIMICA
/
QUIMICA
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
França