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Generation of a human ACTA1-tdTomato reporter iPSC line using CRISPR/Cas9 editing.
Houweling, Peter J; Crossman, Vanessa; Tiong, Chrystal F; Coles, Chantal A; Taylor, Rhonda L; Clayton, Joshua S; Graham, Alison; Vlahos, Katerina; Howden, Sara E; North, Kathryn N.
Afiliação
  • Houweling PJ; Murdoch Children's Research Institute, Melbourne, Victoria, Australia; Department of Pediatrics, The University of Melbourne, Victoria, Australia. Electronic address: Peter.houweling@mcri.edu.au.
  • Crossman V; Murdoch Children's Research Institute, Melbourne, Victoria, Australia.
  • Tiong CF; Murdoch Children's Research Institute, Melbourne, Victoria, Australia.
  • Coles CA; Murdoch Children's Research Institute, Melbourne, Victoria, Australia; Department of Pediatrics, The University of Melbourne, Victoria, Australia.
  • Taylor RL; Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA, Australia; Centre for Medical Research, University of Western Australia, QEII Medical Centre, Nedlands, WA, Australia.
  • Clayton JS; Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA, Australia; Centre for Medical Research, University of Western Australia, QEII Medical Centre, Nedlands, WA, Australia.
  • Graham A; Murdoch Children's Research Institute, Melbourne, Victoria, Australia.
  • Vlahos K; Murdoch Children's Research Institute, Melbourne, Victoria, Australia.
  • Howden SE; Murdoch Children's Research Institute, Melbourne, Victoria, Australia.
  • North KN; Murdoch Children's Research Institute, Melbourne, Victoria, Australia; Department of Pediatrics, The University of Melbourne, Victoria, Australia.
Stem Cell Res ; 75: 103313, 2024 Mar.
Article em En | MEDLINE | ID: mdl-38277710
ABSTRACT
We used gene editing to introduce DNA sequences encoding the tdTomato fluorescent protein into the α -skeletal actin 1 (ACTA1) locus to develop an ACTA1-tdTomato induced pluripotent stem cell reporter line for monitoring differentiation of skeletal muscle. This cell line will be used to better understand skeletal muscle maturation and development in vitro as well as provide a useful tool for drug screening and the evaluation of novel therapeutics for the treatment of skeletal muscle disease.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes Induzidas / Sistemas CRISPR-Cas / Proteína Vermelha Fluorescente Limite: Humans Idioma: En Revista: Stem Cell Res Ano de publicação: 2024 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes Induzidas / Sistemas CRISPR-Cas / Proteína Vermelha Fluorescente Limite: Humans Idioma: En Revista: Stem Cell Res Ano de publicação: 2024 Tipo de documento: Article