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The involvement of Neuregulin-1 in the process of facial nerve injury repair through the utilization of dental pulp stem cells.
Yao, Lihong; Xu, Wanqiu; Liu, Lixue; Xu, Xiaohang; Xi, Hualei; Xue, Bing; Cao, Xiaofang; Lin, Song; Piao, Guiyan; Sun, Jian; Wang, Xiumei.
Afiliação
  • Yao L; Department of Stomatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, China.
  • Xu W; Department of Stomatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, China.
  • Liu L; Department of Stomatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, China.
  • Xu X; Department of Stomatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, China.
  • Xi H; Department of Stomatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, China.
  • Xue B; Department of Stomatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, China.
  • Cao X; Department of Stomatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, China.
  • Lin S; Department of Stomatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, China.
  • Piao G; Department of Stomatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, China.
  • Sun J; Mudanjiang Medical University, Mudanjiang, Heilongjiang Province, China.
  • Wang X; Department of Stomatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, China. hrbwangmei@163.com.
BMC Oral Health ; 24(1): 238, 2024 Feb 14.
Article em En | MEDLINE | ID: mdl-38355448
ABSTRACT

BACKGROUND:

Facial nerve injury often results in poor prognosis due to the challenging process of nerve regeneration. Neuregulin-1, a human calmodulin, is under investigation in this study for its impact on the reparative capabilities of Dental Pulp Stem Cells (DPSCs) in facial nerve injury.

METHODS:

Lentivirus was used to transfect and construct Neuregulin-1 overexpressed DPSCs. Various techniques assessed the effects of Neuregulin-1 osteogenic induction, lipid induction, Reverse Transcription Polymerase Chain Reaction, Western Blot, Cell Counting Kit-8 assay, wound healing, immunofluorescence, Phalloidin staining, nerve stem action potential, Hematoxylin-eosin staining, transmission electron microscopy, and immunohistochemistry.

RESULTS:

Neuregulin-1 effectively enhanced the proliferation, migration, and cytoskeletal rearrangement of DPSCs, while simultaneously suppressing the expression of Ras homolog gene family member A (RhoA) and Microfilament actin (F-actin). These changes facilitated the neural differentiation of DPSCs. Additionally, in vivo experiments showed that Neuregulin-1 expedited the restoration of action potential in the facial nerve trunk, increased the thickness of the myelin sheath, and stimulated axon regeneration.

CONCLUSION:

Neuregulin-1 has the capability to facilitate the repair of facial nerve injuries by promoting the regenerative capacity of DPSCs. Thus, Neuregulin-1 is a significant potential gene in the reparative processes of nerve damage.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traumatismos do Nervo Facial / Polpa Dentária Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: BMC Oral Health Assunto da revista: ODONTOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traumatismos do Nervo Facial / Polpa Dentária Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: BMC Oral Health Assunto da revista: ODONTOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China