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Solobacterium moorei promotes the progression of adenomatous polyps by causing inflammation and disrupting the intestinal barrier.
Yu, Shoujuan; Wang, Xifan; Li, Ziyang; Jin, Dekui; Yu, Mengyang; Li, Jingnan; Li, Yixuan; Liu, Xiaoxue; Zhang, Qi; Liu, Yinghua; Liu, Rong; Wang, Xiaoyu; Fang, Bing; Zhang, Chengying; Wang, Ran; Ren, Fazheng.
Afiliação
  • Yu S; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
  • Wang X; Department of Obstetrics and Gynecology, Columbia University, New York, NY, 10032, USA.
  • Li Z; Key Laboratory of Functional Dairy, Co-Constructed By Ministry of Education and Beijing Government, Department of Nutrition and Health, China Agricultural University, Beijing, 100190, China.
  • Jin D; Department of General Practice, The Third Centers of Chinese PLA General Hospital, Beijing, 100039, China.
  • Yu M; Department of General Practice, The Third Centers of Chinese PLA General Hospital, Beijing, 100039, China.
  • Li J; Department of Gastroenterology, Peking Union Medical College Hospital, Beijing, 100730, China.
  • Li Y; Key Laboratory of Functional Dairy, Co-Constructed By Ministry of Education and Beijing Government, Department of Nutrition and Health, China Agricultural University, Beijing, 100190, China.
  • Liu X; Key Laboratory of Functional Dairy, Co-Constructed By Ministry of Education and Beijing Government, Department of Nutrition and Health, China Agricultural University, Beijing, 100190, China.
  • Zhang Q; Key Laboratory of Functional Dairy, Co-Constructed By Ministry of Education and Beijing Government, Department of Nutrition and Health, China Agricultural University, Beijing, 100190, China.
  • Liu Y; Department of Nutrition, The First Center of Chinese PLA General Hospital, Beijing, 100037, China.
  • Liu R; Key Laboratory of Functional Dairy, Co-Constructed By Ministry of Education and Beijing Government, Department of Nutrition and Health, China Agricultural University, Beijing, 100190, China.
  • Wang X; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
  • Fang B; Key Laboratory of Functional Dairy, Co-Constructed By Ministry of Education and Beijing Government, Department of Nutrition and Health, China Agricultural University, Beijing, 100190, China.
  • Zhang C; Department of General Practice, The Third Centers of Chinese PLA General Hospital, Beijing, 100039, China. zhangchy1969@126.com.
  • Wang R; Key Laboratory of Functional Dairy, Co-Constructed By Ministry of Education and Beijing Government, Department of Nutrition and Health, China Agricultural University, Beijing, 100190, China. wangran@cau.edu.cn.
  • Ren F; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China. renfazheng@cau.edu.cn.
J Transl Med ; 22(1): 169, 2024 02 17.
Article em En | MEDLINE | ID: mdl-38368407
ABSTRACT

BACKGROUND:

Adenomatous polyps (APs) with inflammation are risk factors for colorectal cancer. However, the role of inflammation-related gut microbiota in promoting the progression of APs is unknown.

METHODS:

Sequencing of the 16S rRNA gene was conducted to identify characteristic bacteria in AP tissues and normal mucosa. Then, the roles of inflammation-related bacteria were clarified by Spearman correlation analysis. Furthermore, colorectal HT-29 cells, normal colon NCM460 cells, and azoxymethane-treated mice were used to investigate the effects of the characteristic bacteria on progression of APs.

RESULTS:

The expression levels of inflammation-related markers (diamine oxidase, D-lactate, C-reactive protein, tumor necrosis factor-α, interleukin-6 and interleukin-1ß) were increased, whereas the expression levels of anti-inflammatory factors (interleukin-4 and interleukin-10) were significantly decreased in AP patients as compared to healthy controls. Solobacterium moorei (S. moorei) was enriched in AP tissues and fecal samples, and significantly positively correlated with serum inflammation-related markers. In vitro, S. moorei preferentially attached to HT-29 cells and stimulated cell proliferation and production of pro-inflammatory factors. In vivo, the incidence of intestinal dysplasia was significantly increased in the S. moorei group. Gavage of mice with S. moorei upregulated production of pro-inflammatory factors, suppressed proliferation of CD4+ and CD8+cells, and disrupted the integrity of the intestinal barrier, thereby accelerating progression of APs.

CONCLUSIONS:

S. moorei accelerated the progression of AP in mice via activation of the NF-κB signaling pathway, chronic low-grade inflammation, and intestinal barrier disruption. Targeted reduction of S. moorei presents a potential strategy to prevent the progression of APs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pólipos Adenomatosos / Firmicutes Limite: Animals / Humans Idioma: En Revista: J Transl Med Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pólipos Adenomatosos / Firmicutes Limite: Animals / Humans Idioma: En Revista: J Transl Med Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China