Variation in the affinity of three representative avian adenoviruses for the cellular coxsackievirus and adenovirus receptor.
Vet Res
; 55(1): 23, 2024 Feb 19.
Article
em En
| MEDLINE
| ID: mdl-38374082
ABSTRACT
According to previous studies, three representative avian adenoviral strains utilize coxsackievirus-adenovirus receptor (CAR) as a receptor and seem to exhibit diverse binding affinities and modes. Thus, further revealing the exact molecular mechanism underlying the interaction between different FAdVs and the attachment receptor CAR is necessary. In this study, we successfully solved the crystal structure of the FAdV-4 fiber1 knob at 1.6 Å resolution. The interaction between the fibre knob and different domains of CAR was verified by confocal microscopy, coimmunoprecipitation and surface plasmon resonance (SPR) analysis. The fibre knobs of the three representative fowl adenoviruses specifically recognized CAR domain 1 (D1), but the recognition of CAR domain 2 (D2) by chicken embryo lethal orphan (CELO) strains was weak. These results provide insights into the differences in adenovirusâhost cell interactions and have important implications for the exploration of viral invasion mechanisms.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aviadenovirus
/
Adenovirus A das Aves
Limite:
Animals
Idioma:
En
Revista:
Vet Res
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China