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Velvet antler polypeptide (VAP) protects against cerebral ischemic injury through NF-κB signaling pathway in vitro.
Wu, Qian; Li, Yutao; Ye, Ru; Wang, Hui; Ge, Ying.
Afiliação
  • Wu Q; Physical Examination Center, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan 430074, Hubei Province, China.
  • Li Y; Physical Examination Center, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan 430074, Hubei Province, China. Electronic address: yutao_li0822@163.com.
  • Ye R; Physical Examination Center, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan 430074, Hubei Province, China.
  • Wang H; Physical Examination Center, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan 430074, Hubei Province, China.
  • Ge Y; Physical Examination Center, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan 430074, Hubei Province, China.
J Stroke Cerebrovasc Dis ; 33(5): 107666, 2024 May.
Article em En | MEDLINE | ID: mdl-38423152
ABSTRACT

OBJECTIVE:

Velvet antler polypeptide (VAP) has been shown to play important roles in the immune and nervous systems. The purpose of this study was to investigate the protective effects of VAP on cerebral ischemic injury with the involvement of NF-κB signaling pathway in vitro. MATERIALS AND

METHODS:

PC-12 cells stimulated by oxygen-glucose deprivation/reperfusion (OGD/R) was used to mimic cerebral ischemic injury in vitro. The levels of ROS, SOD, and intracellular concentrations of Ca2+ were measured by the relevant kits. Meanwhile, the expressions of inflammatory cytokines (IL-6, IL-1ß, and TNF-α) were determined by ELISA kit assay. In addition, MTT, EdU, and flow cytometry assays were used to measure the cell proliferation and apoptosis. Besides which, the related proteins of NF-κB signaling pathway were measured by western blotting assay.

RESULTS:

VAP alleviated cerebral ischemic injury by reducing OGD/R-induced oxidative stress, inflammation, and apoptosis in PC-12 cells in a time dependent manner. Mechanistically, VAP inhibited the levels of p-p65 and p-IkB-α in a time dependent manner, which was induced by OGD/R operation. Moreover, NF-κB agonist diprovocim overturned the suppression effects of VAP on OGD/R-induced oxidative stress, inflammation, and apoptosis in PC-12 cells.

CONCLUSIONS:

The results demonstrate that VAP may alleviate cerebral ischemic injury by suppressing the activation of NF-κB signaling pathway.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Chifres de Veado / Traumatismo por Reperfusão Limite: Animals / Humans Idioma: En Revista: J Stroke Cerebrovasc Dis / J. stroke cerebrovasc. dis / Journal of stroke and cerebrovascular diseases Assunto da revista: ANGIOLOGIA / CEREBRO Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Chifres de Veado / Traumatismo por Reperfusão Limite: Animals / Humans Idioma: En Revista: J Stroke Cerebrovasc Dis / J. stroke cerebrovasc. dis / Journal of stroke and cerebrovascular diseases Assunto da revista: ANGIOLOGIA / CEREBRO Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China