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Improvement of survivability and developmental ability in vitrified rat oocytes.
Nakagawa, Yuki; Kaneko, Takehito.
Afiliação
  • Nakagawa Y; Department of Chemistry and Biological Sciences, Faculty of Science and Engineering, Iwate University, Iwate, 020-8551, Japan.
  • Kaneko T; Department of Chemistry and Biological Sciences, Faculty of Science and Engineering, Iwate University, Iwate, 020-8551, Japan; Division of Fundamental and Applied Sciences, Graduate School of Science and Engineering, Iwate University, Iwate, 020-8551, Japan. Electronic address: takekaneko5@gmail.com.
Cryobiology ; 115: 104882, 2024 Jun.
Article em En | MEDLINE | ID: mdl-38452847
ABSTRACT
Oocyte cryopreservation is useful for human fertility treatment and strain preservation in both experimental and domestic animals. However, the embryonic development of vitrified rat oocytes was lower than that of vitrified embryos. To increase the viability of vitrified oocytes, intracellular ice formation during cooling and warming must be prevented. Rapid warming is important to prevent ice formation. Furthermore, suppressing the spontaneous activation of oocytes is also important because vitrification promotes the spontaneous activation of rat oocytes, and thus compromise developmental competence of the gametes. MG132, a proteasome inhibitor, suppresses the spontaneous activation of rat oocytes. Here, we examined the effects of rapid warming and MG132 treatment on the survival and embryonic development of vitrified rat oocytes. The warming rate was adjusted by changing the vitrification solution volume and warming solution temperature. The survival rate of oocytes vitrified in 10 µL solution and warmed at 50 °C (94%) was significantly higher than that of oocytes vitrified in 100 µL and 10 µL solution and warmed at 37 °C (49% and 81%, respectively). Furthermore, the rate of embryonic development of vitrified oocytes treated with MG132 during vitrification, warming, and intracytoplasmic sperm injection (ICSI) (44%) was significantly higher than that of untreated gametes (10%). Offspring were obtained after transferring embryos derived from MG132-treated vitrified oocytes (14%). Altogether, the survivability of vitrified rat oocytes increased by rapid warming, and MG132 improved embryonic development after ICSI.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Criopreservação / Injeções de Esperma Intracitoplásmicas / Desenvolvimento Embrionário / Vitrificação / Leupeptinas Limite: Animals Idioma: En Revista: Cryobiology Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Japão

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Criopreservação / Injeções de Esperma Intracitoplásmicas / Desenvolvimento Embrionário / Vitrificação / Leupeptinas Limite: Animals Idioma: En Revista: Cryobiology Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Japão