VALIDATION OF A NOVEL ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTING PREGNANCY-SPECIFIC PROTEIN B IN MASAI GIRAFFE (GIRAFFA CAMELOPARDALIS TIPPELSKIRCHI).
J Zoo Wildl Med
; 55(1): 42-47, 2024 Mar.
Article
em En
| MEDLINE
| ID: mdl-38453486
ABSTRACT
The Masai giraffe (Giraffa camelopardalis tippelskirchi) is endangered in the wild, and successful reproduction in managed care is important to help maintain assurance populations of this highly charismatic subspecies. Detection of pregnancy in giraffes using hormonal monitoring requires multiple samples and cannot distinguish pregnancy from pseudopregnancy. A novel enzyme-linked immunosorbent assay that can detect pregnancy-specific protein B (PSPB) for pregnancy diagnosis with a single serum sample was developed from a reticulated giraffe (Giraffa camelopardalis reticulata) placenta. Seventy-eight serum samples were analyzed from three female Masai giraffes before and during five gestation periods that resulted in live calf births. Using an optical density cutoff of 0.2, the assay showed a sensitivity of 93% and specificity of 100% for all samples tested. At 59 d of gestation, sensitivity increased to 100%. The earliest pregnancy detection was at 40 d of gestation. This study documents the successful development of a blood-based PSPB assay for pregnancy diagnosis in Masai giraffe, which can help advance conservation efforts in this endangered species.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores Fc
/
Girafas
Limite:
Animals
/
Pregnancy
Idioma:
En
Revista:
J Zoo Wildl Med
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos