Locus-specific detection of pseudouridine with CRISPR-Cas13a.

Sun, Mei; Fang, Xin; Lin, Bingqian; Mo, Jing; Wang, Fang; Zhou, Xiang; Weng, Xiaocheng

Sun, Mei; Fang, Xin; Lin, Bingqian; Mo, Jing; Wang, Fang; Zhou, Xiang; Weng, Xiaocheng.

Afiliação

Sun M; College of Chemistry and Molecular Sciences, Department of Clinical Laboratory of Zhongnan Hospital, Key Laboratory of Biomedical Polymers-Ministry of Education, Wuhan University, Wuhan, Hubei, 430072, P. R. China. xcweng@whu.edu.cn.
Fang X; College of Chemistry and Molecular Sciences, Department of Clinical Laboratory of Zhongnan Hospital, Key Laboratory of Biomedical Polymers-Ministry of Education, Wuhan University, Wuhan, Hubei, 430072, P. R. China. xcweng@whu.edu.cn.
Lin B; College of Chemistry and Molecular Sciences, Department of Clinical Laboratory of Zhongnan Hospital, Key Laboratory of Biomedical Polymers-Ministry of Education, Wuhan University, Wuhan, Hubei, 430072, P. R. China. xcweng@whu.edu.cn.
Mo J; College of Chemistry and Molecular Sciences, Department of Clinical Laboratory of Zhongnan Hospital, Key Laboratory of Biomedical Polymers-Ministry of Education, Wuhan University, Wuhan, Hubei, 430072, P. R. China. xcweng@whu.edu.cn.
Wang F; Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China.
Zhou X; College of Chemistry and Molecular Sciences, Department of Clinical Laboratory of Zhongnan Hospital, Key Laboratory of Biomedical Polymers-Ministry of Education, Wuhan University, Wuhan, Hubei, 430072, P. R. China. xcweng@whu.edu.cn.
Weng X; Wuhan TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan, Hubei 430071, P. R. China.

Chem Commun (Camb) ; 60(30): 4088-4091, 2024 Apr 09.

Article em En | MEDLINE | ID: mdl-38511312

ABSTRACT

ABSTRACT

We combined the CRISPR-Cas13a system with CMC chemical labeling, developing an approach that enables precise identification of pseudouridine (Ψ) sites at specific loci within ribosomal RNA (rRNA), messenger RNA (mRNA) and small nuclear RNAs (snRNA). This method, with good efficiency and simplicity, detects Ψ sites through fluorescence measurement, providing a straightforward and fast validation for targeted Ψ sites of interest.

Assuntos

Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas; Pseudouridina; Pseudouridina/genética; RNA Nuclear Pequeno/genética; RNA Ribossômico; RNA Mensageiro/genética

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudouridina / Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2024 Tipo de documento: Article

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