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Hypoxic preconditioning accelerates the healing of ischemic intestinal injury by activating HIF-1α/PPARα pathway-mediated fatty acid oxidation.
Li, Linxia; Liu, Yanqi; Zhi, Na; Ji, Yaoxuan; Xu, Jialing; Mao, Guoyun; Wang, Yazhou; Ma, Jin; Wang, Yunying.
Afiliação
  • Li L; Department of Aerospace Medicine, Air Force Medical University, 710032, Xi'an, China.
  • Liu Y; Department of Aerospace Medicine, Air Force Medical University, 710032, Xi'an, China.
  • Zhi N; Department of Aerospace Medicine, Air Force Medical University, 710032, Xi'an, China.
  • Ji Y; Department of Aerospace Medicine, Air Force Medical University, 710032, Xi'an, China.
  • Xu J; Department of Aerospace Medicine, Air Force Medical University, 710032, Xi'an, China.
  • Mao G; Department of Aerospace Medicine, Air Force Medical University, 710032, Xi'an, China.
  • Wang Y; Department of Neurobiology and Institute of Neurosciences, Air Force Medical University, 710032, Xi'an, China.
  • Ma J; Department of Aerospace Medicine, Air Force Medical University, 710032, Xi'an, China. jin-ma@fmmu.edu.cn.
  • Wang Y; Department of Aerospace Medicine, Air Force Medical University, 710032, Xi'an, China. yunyingwang@fmmu.edu.cn.
Cell Death Discov ; 10(1): 164, 2024 Apr 04.
Article em En | MEDLINE | ID: mdl-38575595
ABSTRACT
Hypoxic preconditioning (HPC) has been shown to improve organ tolerance to subsequent severe hypoxia or ischemia. However, its impact on intestinal ischemic injury has not been well studied. In this study, we evaluated the effects of HPC on intestinal ischemia in rats. Intestinal rehabilitation, levels of fatty acid oxidation (FAO) by-products, intestinal stem cells (ISCs), levels of hypoxia-inducible factor 1 subunit α (HIF-1α) and its downstream genes such as peroxisome proliferator-activated receptor α (PPARα), and carnitine palmitoyltransferase 1a (CPT1A) were assessed at distinct time intervals following intestinal ischemia with or without the interference of HIF-1α. Our data showed that HPC facilitates the restoration of the intestinal structure and enhances the FAO, by boosting intestinal stem cells. Additionally, HIF-1α, PPARα, and CPT1A mRNA and their protein levels were generally up-regulated in the small intestine of HPC rats as compared to the control group. Our vitro experiment also shows low-oxygen induces highly levels of HIF-1α and its downstream genes, with a concurrent increase in FAO products in IEC-6 cells. Furthermore, the above phenomenon could be reversed by silencing HIF-1α. In conclusion, we hypothesize that HPC can stimulate the activation of intestinal stem cells via HIF-1α/PPARα pathway-mediated FAO, thereby accelerating the healing process post ischemic intestinal injury.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Cell Death Discov Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Cell Death Discov Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China