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Ontogenetic Expression of Aquaporins in the Kidney and Urinary Bladder of the Japanese Tree Frog, Dryophytes japonicus.
Hibino, Masatoshi; Aoki, Ryota; Ha, Duy Anh; Sano, Haruna; Yamashita, Shiori; Ogasawara, Haruto; Nishio, Kazuma; Kotake, Kohei; Uddin Mamun, Md Main; Okada, Reiko; Shibata, Yuki; Suzuki, Masakazu.
Afiliação
  • Hibino M; Department of Biological Science, Faculty of Science, Shizuoka University, Shizuoka 422-8529, Japan.
  • Aoki R; Department of Biological Science, Faculty of Science, Shizuoka University, Shizuoka 422-8529, Japan.
  • Ha DA; Department of Science, Graduate School of Integrated Science and Technology, Shizuoka University, Shizuoka 422-8529, Japan.
  • Sano H; Department of Biological Science, Faculty of Science, Shizuoka University, Shizuoka 422-8529, Japan.
  • Yamashita S; Department of Biological Science, Faculty of Science, Shizuoka University, Shizuoka 422-8529, Japan.
  • Ogasawara H; Department of Biological Science, Faculty of Science, Shizuoka University, Shizuoka 422-8529, Japan.
  • Nishio K; Department of Science, Graduate School of Integrated Science and Technology, Shizuoka University, Shizuoka 422-8529, Japan.
  • Kotake K; Department of Science, Graduate School of Integrated Science and Technology, Shizuoka University, Shizuoka 422-8529, Japan.
  • Uddin Mamun MM; Integrated Bioscience Section, Graduate School of Science and Technology, Shizuoka University, Shizuoka 422-8529, Japan.
  • Okada R; Department of Biological Science, Faculty of Science, Shizuoka University, Shizuoka 422-8529, Japan.
  • Shibata Y; Department of Science, Graduate School of Integrated Science and Technology, Shizuoka University, Shizuoka 422-8529, Japan.
  • Suzuki M; Integrated Bioscience Section, Graduate School of Science and Technology, Shizuoka University, Shizuoka 422-8529, Japan.
Zoolog Sci ; 41(1): 124-131, 2024 Feb.
Article em En | MEDLINE | ID: mdl-38587525
ABSTRACT
For adult anuran amphibians, the kidney and urinary bladder play important osmoregulatory roles through water reabsorption. In the present study, we have examined ontogenetic expression of aquaporins, i.e., AQP2, AQPamU (AQP6ub, AQPa2U), and AQP3, in these organs using the Japanese tree frog, Dryophytes japonicus. Immunohistochemistry using the metamorphosing larvae at stages 40-43 localized AQP2 protein to the collecting ducts in the dorsal zone of the mesonephric kidney. At prometamorphic stages 40 and 41, labelling of AQP2 protein was observed in the apical/ subapical regions of the collecting duct cells. At climax stages 42 and 43, labels for AQP2 and AQP3 became observed in the apical/subapical regions and basolateral membrane of the collecting duct cells, respectively, as seen in the adults. As for the urinary bladder, immuno-positive labels for AQPamU were localized to the apical/subapical regions of granular cells in the mucosal epithelium at stages 40-43. On the other hand, AQP3 immunoreactivity was hardly observed in the urinary bladder at stage 40, and weakly appeared in many granular cells at stage 41. Thereafter, labels for AQP3 became evident along the basolateral membrane of granular cells at stages 42 and 43, together with AQPamU in the apical/subapical regions. These results suggest that the kidney and urinary bladder might be capable of water reabsorption, via AQP2, AQPamU, and AQP3, at stage 42, contributing to the acclimation of the tree frogs to terrestrial environments.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bexiga Urinária / Aquaporina 2 Limite: Animals País/Região como assunto: Asia Idioma: En Revista: Zoolog Sci Assunto da revista: BIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Japão

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bexiga Urinária / Aquaporina 2 Limite: Animals País/Região como assunto: Asia Idioma: En Revista: Zoolog Sci Assunto da revista: BIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Japão