[Bioinformatics-based analysis of the effect of general Transcription Factor IIH on prognosis of Prostate cancer].
Zhonghua Yi Xue Za Zhi
; 104(16): 1410-1417, 2024 Apr 23.
Article
em Zh
| MEDLINE
| ID: mdl-38644292
ABSTRACT
Objective:
To investigate the genetic and expression characteristics of transcription factor IIH (TFIIH) in pre-initiationcomplex in prostate cancer (PCa) and its relationship with prostate cancer progression.Methods:
Analyzing the expression characteristics and clinical signification of TFIIH subunits about 495 cases of PCa and 52 cases of adjacent cancer in The Cancer Genome Atlas-Prostate adenocarcinoma (TCGA-PRAD) database. PCa microarray chip was used to verify the correlation between the key factor General Transcription Factor IIH Subunit 4 (GTF2H4) in TFIIH and clinical features.Results:
The 495 patients with PCa were (61.01±6.82) years old.The mRNA expression of ERCC3ãGTF2H4 and MNAT1 were high in PCa tissues with GS≥8(P<0.05). The expression of GTF2H4 and MNAT1 were relevant to the pathological stages(P<0.05). High expression of GTF2H4 has higher biochemical recurrence (BCR) rate in PCa patients(HR=2.47, 95%CI1.62-3.77, P<0.001), which has better predictive effect of BCR in PCa patients(The 3rd, 5th, and 7th year AUC all>0.7) than other subunits, and it has been verified in four additional databases. Single-factor Cox regression analysis showed that GTF2H4 were risk factors for BCR (HR=2.470, 95%CI1.620-3.767, P<0.001) and GTF2H5 were protective factors(HR=0.506,95%CI 0.336-0.762, P=0.001). The results of immunohistochemical staining showed that the protein expression of GTF2H4 was correlated with the clinical features of PCa patients.The differences of the above results were statistically significant.Conclusion:
GTF2H4, the key factor of TFIIH, is highly expressed in PCa and indicates a poor prognosis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Próstata
/
Biologia Computacional
Limite:
Aged
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Humans
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Male
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Middle aged
Idioma:
Zh
Revista:
Zhonghua Yi Xue Za Zhi
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China