Thymoquinone ameliorates symptoms of Parkinson's disease in a 6-OHDA rat model by downregulation of miR-204-3p.
Behav Pharmacol
; 35(4): 201-210, 2024 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-38660812
ABSTRACT
microRNAs (miRNAs) play a significant role in the pathophysiology of Parkinson's disease. In this study, we evaluated the neuroprotective effect of thymoquinone on the expression profiles of miRNA and cognitive functions in the 6-hydroxydopamine (6-OHDA)-induced Parkinson's model. Male adult Wistar albino rats (200-230â
g, n â
=â
36) were randomly assigned to six groups Sham, thymoquinone (10â
mg/kg, p.o.), 6-OHDA, 6-OHDAâ
+â
thymoquinone (10â
mg/kg), 6-OHDAâ
+â
thymoquinone (20â
mg/kg), and 6-OHDAâ
+â
thymoquinone (50â
mg/kg). Behavioral changes were detected using the open field and the elevated plus maze tests. The mature 728 miRNA expressions were evaluated by miRNA microarray (GeneChip miRNA 4.0). Ten miRNAs were selected (rno-miR-212-5p, rno-miR-146b-5p, rno-miR-150-5p, rno-miR-29b-2-5p, rno-miR-126a-3p, rno-miR-187-3p, rno-miR-34a-5p, rno-miR-181d-5p, rno-miR-204-3p, and rno-miR-30c-2-3p) and confirmed by real-time PCR. Striatum samples were stained with hematoxylin-eosin to determine the effect of dopaminergic lesions. One-way ANOVA test and independent sample t -test were used for statistical analyses. rno-miR-204-3p was upregulated at 6-OHDA and downregulated at the 50â
mg/kg dose of thymoquinone. In conclusion, thymoquinone at a dose of 50â
mg/kg ameliorates symptoms of Parkinson's disease in a 6-OHDA rat model by downregulation of miR-204-3p. Also, the results showed that thymoquinone can improve locomotor activity and willing exploration and decreased anxiety. Therefore, thymoquinone can be used as a therapeutic agent.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doença de Parkinson
/
Regulação para Baixo
/
Benzoquinonas
/
Oxidopamina
/
MicroRNAs
Limite:
Animals
Idioma:
En
Revista:
Behav Pharmacol
Assunto da revista:
CIENCIAS DO COMPORTAMENTO
/
FARMACOLOGIA
Ano de publicação:
2024
Tipo de documento:
Article