Your browser doesn't support javascript.
loading
Human T-Cell Responses to Metallic Ion-Doped Bioactive Glasses.
Abreu, Hugo; Lallukka, Mari; Miola, Marta; Spriano, Silvia; Vernè, Enrica; Raineri, Davide; Leigheb, Massimiliano; Ronga, Mario; Cappellano, Giuseppe; Chiocchetti, Annalisa.
Afiliação
  • Abreu H; Department of Health Sciences, Interdisciplinary Research Center of Autoimmune Diseases-IRCAD, Università del Piemonte Orientale, 28100 Novara, Italy.
  • Lallukka M; Center for Translational Research on Autoimmune and Allergic Diseases-CAAD, Università del Piemonte Orientale, 28100 Novara, Italy.
  • Miola M; Applied Science and Technology Department, Politecnico di Torino, 10129 Torino, Italy.
  • Spriano S; Applied Science and Technology Department, Politecnico di Torino, 10129 Torino, Italy.
  • Vernè E; Applied Science and Technology Department, Politecnico di Torino, 10129 Torino, Italy.
  • Raineri D; Applied Science and Technology Department, Politecnico di Torino, 10129 Torino, Italy.
  • Leigheb M; Department of Health Sciences, Interdisciplinary Research Center of Autoimmune Diseases-IRCAD, Università del Piemonte Orientale, 28100 Novara, Italy.
  • Ronga M; Center for Translational Research on Autoimmune and Allergic Diseases-CAAD, Università del Piemonte Orientale, 28100 Novara, Italy.
  • Cappellano G; Department of Health Sciences, Interdisciplinary Research Center of Autoimmune Diseases-IRCAD, Università del Piemonte Orientale, 28100 Novara, Italy.
  • Chiocchetti A; Orthopaedics and Traumatology Unit, "Maggiore della Carità" Hospital, 28100 Novara, Italy.
Int J Mol Sci ; 25(8)2024 Apr 19.
Article em En | MEDLINE | ID: mdl-38674086
ABSTRACT
Biomaterials are extensively used as replacements for damaged tissue with bioactive glasses standing out as bone substitutes for their intrinsic osteogenic properties. However, biomaterial implantation has the following risks the development of implant-associated infections and adverse immune responses. Thus, incorporating metallic ions with known antimicrobial properties can prevent infection, but should also modulate the immune response. Therefore, we selected silver, copper and tellurium as doping for bioactive glasses and evaluated the immunophenotype and cytokine profile of human T-cells cultured on top of these discs. Results showed that silver significantly decreased cell viability, copper increased the T helper (Th)-1 cell percentage while decreasing that of Th17, while tellurium did not affect either cell viability or immune response, as evaluated via multiparametric flow cytometry. Multiplex cytokines assay showed that IL-5 levels were decreased in the copper-doped discs, compared with its undoped control, while IL-10 tended to be lower in the doped glass, compared with the control (plastic) while undoped condition showed lower expression of IL-13 and increased MCP-1 and MIP-1ß secretion. Overall, we hypothesized that the Th1/Th17 shift, and specific cytokine expression indicated that T-cells might cross-activate other cell types, potentially macrophages and eosinophils, in response to the scaffolds.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Citocinas / Vidro Limite: Humans Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Itália

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Citocinas / Vidro Limite: Humans Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Itália