Your browser doesn't support javascript.
loading
Effects of regulatory B cells on intracranial aneurysms by mediating IL-1ß/IL-1R pathways.
Shi, Chanhong; Tian, Fang; Yang, Xuewei; Song, Jinhui; Qi, Dongwang; Li, Jianwei.
Afiliação
  • Shi C; Department of Neurology, Yiwu Hospital Affiliated to Wenzhou Medical University, Yiwu Central Hospital. Yiwu 322000, China. chanhongs@163.com.
  • Tian F; Jiangsu Key Laboratory of Experimental & Translational Non-coding RNA Research, Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou, 225001, China. fangtiantf@163.com.
  • Yang X; Department of Neurology, Yiwu Hospital Affiliated to Wenzhou Medical University, Yiwu Central Hospital. Yiwu 322000, China. yxw15958929950@163.com.
  • Song J; Department of Neurology, Yiwu Hospital Affiliated to Wenzhou Medical University, Yiwu Central Hospital. Yiwu 322000, China. 240383387@qq.com.
  • Qi D; Department of Neurology, Yiwu Hospital Affiliated to Wenzhou Medical University, Yiwu Central Hospital. Yiwu 322000, China. yiwuqdw@163.com.
  • Li J; Department of Neurosurgery, Yiwu Hospital Affiliated to Wenzhou Medical University, Yiwu Central Hospital. Yiwu 322000, China. ljw84131421@sina.com.
Cell Mol Biol (Noisy-le-grand) ; 70(4): 152-157, 2024 Apr 28.
Article em En | MEDLINE | ID: mdl-38678612
ABSTRACT
The purpose of this study was to explore the effects of regulatory B-cells (Breg) on intracranial aneurysms by mediating IL-1ß/IL-1R pathways.  The study involved 60 patients undergoing angiography in a hospital from January to June 2022, divided into two groups 30 with intracranial aneurysms (observation group) and 30 without (control group). Researchers extracted peripheral blood mononuclear cells (PBMC) to analyze the proportion of CD19+CD24hiCD38hiB cells using flow cytometry. These cells, along with T-cells and regulatory T-cells (Treg), were isolated through magnetic bead cell sorting. Following co-culture, the proliferation of T-cells and their related secretory factors were assessed. Additionally, Breg cells, treated with an IL-1R receptor blocker or IL-1R expression adenovirus, were studied to evaluate the levels of IL-10 and TGF-ß. In the study, the observation group showed lower levels of CD19+CD24hiCD38hiB cells, IL-10, and TGF-ß in PBMC than the control group (P<0.05). T-cell proportions were similar in both groups pre and post co-culture (P>0.05). Post co-culture, IFN-γ decreased while IL-4 increased in both groups. The observation group had higher IFN-γ and lower IL-4 than the control group (P<0.05). TNF-α in CD8+T cells, and granzyme B and perforin mRNA levels decreased post co-culture but were higher in the observation group (P<0.05). IL-10 and TGF-ß in Treg cells increased in both groups post co-culture but were lower in the observation group (P<0.05). The observation group also had fewer CD19+IL-1R+IL-10+B cells (P<0.05). After IL-1R blocker addition, IL-10 and TGF-ß in the supernatant decreased in the observation group (P<0.05). Following transfection, IL-1 and TGF-ß levels increased compared to the blank group (P<0.05). The function of peripheral blood CD19+CD24hiCD38hiB cells is impaired in patients with intracranial aneurysms, which may be related to IL-1ß/IL-1R pathways disorder.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aneurisma Intracraniano / Receptores de Interleucina-1 / Interleucina-1beta / Linfócitos B Reguladores Limite: Female / Humans / Male Idioma: En Revista: Cell Mol Biol (Noisy-le-grand) Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aneurisma Intracraniano / Receptores de Interleucina-1 / Interleucina-1beta / Linfócitos B Reguladores Limite: Female / Humans / Male Idioma: En Revista: Cell Mol Biol (Noisy-le-grand) Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China