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Trim66's paternal deficiency causes intrauterine overgrowth.
Mielnicka, Monika; Tabaro, Francesco; Sureka, Rahul; Acurzio, Basilia; Paoletti, Renata; Scavizzi, Ferdinando; Raspa, Marcello; Crevenna, Alvaro H; Lapouge, Karine; Remans, Kim; Boulard, Matthieu.
Afiliação
  • Mielnicka M; https://ror.org/01yr73893 Epigenetics and Neurobiology Unit, EMBL Rome, European Molecular Biology Laboratory, Monterotondo, Italy.
  • Tabaro F; https://ror.org/01yr73893 Epigenetics and Neurobiology Unit, EMBL Rome, European Molecular Biology Laboratory, Monterotondo, Italy.
  • Sureka R; https://ror.org/01yr73893 Epigenetics and Neurobiology Unit, EMBL Rome, European Molecular Biology Laboratory, Monterotondo, Italy.
  • Acurzio B; https://ror.org/01yr73893 Epigenetics and Neurobiology Unit, EMBL Rome, European Molecular Biology Laboratory, Monterotondo, Italy.
  • Paoletti R; Plaisant s.r.l., Rome, Italy.
  • Scavizzi F; National Research Council (IBBC), CNR-Campus International Development (EMMA-INFRAFRONTIER-IMPC), Monterotondo, Italy.
  • Raspa M; National Research Council (IBBC), CNR-Campus International Development (EMMA-INFRAFRONTIER-IMPC), Monterotondo, Italy.
  • Crevenna AH; https://ror.org/01yr73893 Epigenetics and Neurobiology Unit, EMBL Rome, European Molecular Biology Laboratory, Monterotondo, Italy.
  • Lapouge K; https://ror.org/01yr73893 European Molecular Biology Laboratory, Protein Expression and Purification Core Facility, Heidelberg, Germany.
  • Remans K; https://ror.org/01yr73893 European Molecular Biology Laboratory, Protein Expression and Purification Core Facility, Heidelberg, Germany.
  • Boulard M; https://ror.org/01yr73893 Epigenetics and Neurobiology Unit, EMBL Rome, European Molecular Biology Laboratory, Monterotondo, Italy matthieu.boulard@embl.it.
Life Sci Alliance ; 7(7)2024 Jul.
Article em En | MEDLINE | ID: mdl-38719749
ABSTRACT
The tripartite motif-containing protein 66 (TRIM66, also known as TIF1-delta) is a PHD-Bromo-containing protein primarily expressed in post-meiotic male germ cells known as spermatids. Biophysical assays showed that the TRIM66 PHD-Bromodomain binds to H3 N-terminus only when lysine 4 is unmethylated. We addressed TRIM66's role in reproduction by loss-of-function genetics in the mouse. Males homozygous for Trim66-null mutations produced functional spermatozoa. Round spermatids lacking TRIM66 up-regulated a network of genes involved in histone acetylation and H3K4 methylation. Profiling of H3K4me3 patterns in the sperm produced by the Trim66-null mutant showed minor alterations below statistical significance. Unexpectedly, Trim66-null males, but not females, sired pups overweight at birth, hence revealing that Trim66 mutations cause a paternal effect phenotype.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Histonas Limite: Animals Idioma: En Revista: Life Sci Alliance Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Itália

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Histonas Limite: Animals Idioma: En Revista: Life Sci Alliance Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Itália