Mesenchymal Stem Cells from the Deciduous Tooth Pulp Lose their Ability to Suppress the Differentiation of Dendritic Cells during Long-Term Culturing.
Bull Exp Biol Med
; 176(5): 672-679, 2024 Mar.
Article
em En
| MEDLINE
| ID: mdl-38733483
ABSTRACT
A culture of cells expressing markers of mesenchymal stem cells (MSC) (CD73, CD90, CD44, CD29, and CD49b), but not hematopoietic cell markers, and capable of multilineage differentiation was isolated from the deciduous tooth pulp. Co-culturing with immature dendritic cells in the presence of LPS did not reveal an ability of the MSC to suppress the maturation of dendritic cells. On the contrary, co-culturing of MSC with monocytes in the presence of granulocyte-macrophage CSF and IL-4 led to complete suppression of monocyte differentiation into dendritic cells. However, long-term culturing of MSC from dental pulp showed that by the passage 11, they almost completely lose their suppressor ability. These results indicate that the immunological properties of MSC can change during culturing without changing their phenotypic markers. This should be taken into account when creating biomedical cell products.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dente Decíduo
/
Células Dendríticas
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Diferenciação Celular
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Técnicas de Cocultura
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Polpa Dentária
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Células-Tronco Mesenquimais
Limite:
Humans
Idioma:
En
Revista:
Bull Exp Biol Med
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Federação Russa