Respiratory and Metabolic Responses of CD4 + T Cells to Acute Exercise and Their Association with Cardiorespiratory Fitness.
Med Sci Sports Exerc
; 56(10): 1882-1892, 2024 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-38768035
ABSTRACT
INTRODUCTION:
The study aimed to investigate to what extent acute endurance exercise, especially eccentric exercise and cardiorespiratory fitness, affects the metabolic profile of CD4 + cells.METHODS:
Fifteen male, healthy adults aged between 20 and 33 yr with a maximal oxygen uptake (VÌO 2max ) between 44 and 63 mL·kg -1 ·min -1 performed a downhill run (DR) and a level run (LR) for 45 min at 70% of their VÌO 2max on a treadmill in a crossover design. Blood samples were taken before (T0), directly after (T1), 3 h after (T3), and 24 h (T24) after each exercise for analyzing leukocyte numbers and cytokine levels. Isolated CD4 + cells were incubated for 4 h in autologous resting versus 3 h after exercise serum (T3 DR and T3 LR), and subsequently, cellular respiration, transcriptomic, and metabolomics profiles were measured.RESULTS:
The systemic immune inflammation index increased significantly after DR and LR at T1 and T3 ( P < 0.001). In contrast, the transcriptomic and metabolic profile of CD4 + cells showed no significant alterations after incubation in T3 exercise serum. However, cardiorespiratory fitness positively correlated with the maximal mitochondrial respiration in CD4 + cells after incubation with T3 LR serum ( r = 0.617, P = 0.033) and with gene expression of oxidative phosphorylation and levels of different metabolites. Similarly, VÌO 2max was associated with an anti-inflammatory profile on RNA level. Lower lactate, methylmalonic acid, and d -gluconic acid levels were found in CD4 + cells of participants with a high VÌO 2max ( P < 0.001).CONCLUSIONS:
Acute exercise leads to a mild proinflammatory milieu with only small changes in the metabolic homeostasis of CD4 + cells. High cardiorespiratory fitness is associated with a metabolic shift to oxidative phosphorylation in CD4 + cells. Functional relevance of this metabolic shift needs to be further investigated.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Consumo de Oxigênio
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Linfócitos T CD4-Positivos
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Estudos Cross-Over
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Aptidão Cardiorrespiratória
Limite:
Adult
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Humans
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Male
Idioma:
En
Revista:
Med Sci Sports Exerc
/
Med. sci. sports exerc
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Medicine and science in sports and exercise
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Alemanha