A simplified and efficient method for single-step, targeted gene deletion in mycobacteria.
Tuberculosis (Edinb)
; 147: 102520, 2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-38781657
ABSTRACT
Targeted gene deletion in mycobacteria remain complicated, requiring expertise and multiple steps. Here we present a single-step, easy to understand and perform method for targeted gene deletion. Using this method, we successfully deleted several genes in both M. smegmatis and M. abscessus. We believe this method will facilitate molecular research of mycobacteria and make it accessible to a greater number of researchers throughout the world.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Deleção de Genes
/
Mycobacterium smegmatis
Limite:
Humans
Idioma:
En
Revista:
Tuberculosis (Edinb)
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Israel