Clinical and pathologic characterization of a mouse model of graded limbal stem cell deficiency.
Exp Eye Res
; 244: 109942, 2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-38795839
ABSTRACT
Limbal stem cell deficiency (LSCD) is a clinically challenging eye disease caused by damage to limbal stem cells (LSCs). Currently, the international consensus classifies LSCD into three clinical stages based on the disease severity. However, no existing animal models attempt to replicate the varying degrees of LSCD observed in clinical cases. The present study demonstrates an easy-to-create, reproducible, and reliable mouse model of graded LSCD. To achieve mild, moderate, or severe LSCD, filter paper rings with a variety of central angles (90°, 180°, or 270°) are utilized to deliver alkali burns to different sizes of the limbal area (1, 2, or 3 quarters). The animal model has successfully resulted in the development of clinical signs and pathological manifestations in escalating severity that are similarly observed in the three clinical stages of LSCD. Our study thus provides new insights into distinct pathological features underlying different grades of LSCD and serves as a new tool for further exploring the disease mechanisms and developing new effective therapeutics for repairing damaged LSCs.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Queimaduras Químicas
/
Queimaduras Oculares
/
Limbo da Córnea
/
Doenças da Córnea
/
Modelos Animais de Doenças
Limite:
Animals
Idioma:
En
Revista:
Exp Eye Res
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China